Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with isopropanolamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented peer-reviewed publication.

Data source

Reference

Reference Type:

publication

Title:

Reduction of aquatic toxicity of linear alkylbenzene sulfonate (LAS) by biodegradation

Author:

Kimerle, R.A. and Swisher, R.D.

Year:

1977

Bibliographic source:

Water Research 11:31-37

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009 1975. Method for acute toxicity tests with fish, macroinvertebrates and amphibians.with fish, macroinvertebrates and amphibians.

Principles of method if other than guideline:

Acute toxicity tests were conducted on high molecular weight LAS, individual pure homologues, non-linear LAS components (dialkyl tetralin or indane sulfonates (DTIS), and model biodegradation intermediates (sulfophenyl undecane, SØU) in order to determine whether biodegradation decreases toxicity. In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three beakers. No food was added for the duration of the test.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

EC Number:

270-115-0

EC Name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

Cas Number:

68411-30-3

Molecular formula:

Not available - not a single isomer - see remarks

IUPAC Name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

Details on test material:

1) High molecular weight LAS: Average chain length = 13.3; C11 1%, C12 8%, C13 52%, C14 39%
2) Individual LAS homologues of C10, C11, C12, C13, and C14
3) Nonlinear LAS components (DTIS)
4) Model biodegradation intermediates

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three replicate beakers per concentration. Five concentrations were tested.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Crustacea

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

open system

Test conditions

Hardness:

250 mg/L

Test temperature:

not reported

pH:

not reported

Dissolved oxygen:

not reported

Reference substance (positive control):

not required

Results and discussion

Any other information on results incl. tables

		Daphnia magna
	Average chain length	24 hour	48 hour
High molecular weight LAS	13.3	2.6 ± 0.1	2.3 ± 0.1
Individual homologues LAS
C10	10	53.1 ± 0.4	12.3 ± 2.6
C11	11	15.8 ± 3.0	5.7 ± 0.6
C12	12	10.7 ± 1.6	3.5 ± 1.0
C13	13	2.7 ± 0.4	2.0 ± 0.3
C14	14	1.2 ± 0.2	0.7 ± 0.2
Nonlinear LAS components (DTIS)
C10	10	106.0 ± 27.0	98.0 ± 21.3
C12	12	55.1 ± 9.1	34.1 ± 5.1
C14	14	12.4 ± 1.4	10.0 ± 1.0
Model biodegradation intermediates
C4 (SØ Butyrate)	4	~12,000	~6,000
C5 (SØ Valerate)	5	~12,000	~5,000
C11 (SØU)	11	355 ± 150*	208 ± 85*

*Subsequent repurification of this sample yielded a product with the same isomeric composition but with LC₅₀ values over 1000 mg/L for daphnids (Swisher et al., 1976).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Partial biodegradation of LAS is shown to significantly reduce the specific toxicity of the remaining LAS to Daphnia magna.

Executive summary:

The toxicity of high molecular weight LAS, individual LAS homologues, and nonlinear LAS components (DTIS) was measured in a series of aquatic toxicity tests with Daphnia magna. Results show that biodegradation of LAS influences the toxicity, with the remaining LAS becoming less toxic. The 48 -hour LC50 of high molecular weight LAS to Daphnia magna is 2.4 mg/L.