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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Remarks:
O2 consumption
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
other: Method B-21
Principles of method if other than guideline:
O2 consumption test
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
no
Details on sampling:
not applicable
Vehicle:
not specified
Details on test solutions:
no data
Test organisms (species):
activated sludge
Details on inoculum:
no data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Remarks on exposure duration:
none
Post exposure observation period:
not applicable
Hardness:
no data
Test temperature:
no data
pH:
no data
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
Nominal: 10, 32 and 100 mg/L
Details on test conditions:
no data
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
90 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: O2 consumption
Details on results:
none
Results with reference substance (positive control):
3h IC50 = 18 mg/L based on 02 consumption
Reported statistics and error estimates:
not applicable

 Test-Substance (mg/L)

 O2 consumption

 % Inhibition

 100

 0.32

 50.8

 32

 0.36

 44.7

 10

 0.36

 44.7

Reference substance : 3,5-Dichlorphenol

 Test-Substance (mg/L)

 O2 consumption

 % Inhibition

 100

 0.08

 87.7

 32

 0.10

 84.7

 10

 0.56

 13.9
Validity criteria fulfilled:
not specified
Conclusions:
The 3 h IC50 for the test substance was found to be 90 mg/L (nominal).
Executive summary:

A study was conducted according to Method B-21 to determine the toxicity of the test substance (at ca. 80.5% purity) to activated sludge after an exposure of 3 h at concentration 10, 32 and 100 mg/L. Toxicity was evaluated based on reduction of O2 consumption. The reference substance 3, 5 -dichlorophenol was tested in parallel.

The 3 h IC50 for the test substance was found to be 90 mg/L (nominal). The 3 h IC50 for the reference substance was found to be 18 mg/L.

Description of key information

The study selected as key study was conducted to determine the toxicity of the test substance (at ca. 80.5 % purity) to activated sludge after an exposure of 3 h at concentration 10, 32 and 100 mg/L. Toxicity was evaluated based on reduction of O2 consumption. The reference substance 3, 5 -dichlorophenol was tested in parallel.

The 3 h IC50 for the test substance was equivalent to 90 mg/L (nominal). The 3 h IC50 for the reference substance was 18 mg/L.

Other supporting studies results are available, the results of which are presented below:

FAT 20003/H : 3h IC50 : . 400 mg/L

FAT 20003/G : 21d IC50 : > 300 mg/L.

FAT 20003/F : 21d IC50 : > 300 mg/L.

FAT 20003/D : 21d IC50 : > 300 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
90 mg/L

Additional information