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EC number: 219-210-0 | CAS number: 2387-03-3
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Assessment of the inhibitory effect of a test substance LUMOGEN GB 0790 ST. BER. 100% (chemical name: 2-hydroxynaphthalene-1-carbaldehyde [(2-hydroxy-1-naphthyl)methylene]hydrazone) on the oxygen consumption rate of aerobic micro-organisms (activated sludge) after short-term exposure (30 or 180min) was conducted.
The effective concentration i.e EC20; EC50 and EC80 in the activated sludge respiration inhibition test when mciroorganisms were exposed to LUMOGEN GB 0790 ST. BER. 100% (Chemical name: 2-hydroxynaphthalene-1-carbaldehyde [(2-hydroxy-1-naphthyl)methylene]hydrazone) was found to be >1000 mg/L during test duration of 180 minutes.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Toxicity to micro-organism study for Target CAS no. 2387-03-3 - C.I. Pigment Yellow 101: Summarized as fallowed:
Study report (Sustainability Support Service (Europe) AB, report no. 01/0031/50/1, 2001): Assessment of the inhibitory effect of a test substance LUMOGEN GB 0790 ST. BER. 100% (chemical name: 2-hydroxynaphthalene-1-carbaldehyde [(2-hydroxy-1-naphthyl)methylene]hydrazone) on the oxygen consumption rate of aerobic micro-organisms (activated sludge) after short-term exposure (30 or 180min) was conducted.
The effective concentration i.e EC20; EC50 and EC80 in the activated sludge respiration inhibition test when mciroorganisms were exposed to LUMOGEN GB 0790 ST. BER. 100% (Chemical name: 2-hydroxynaphthalene-1-carbaldehyde [(2-hydroxy-1-naphthyl)methylene]hydrazone) was found to be >1000 mg/L during test duration of 180 minutes.
In a supporting study of author Vanya B. Kurteva et. al, 2011: Toxicity to micro-organisms study was conducted on 10 different gram positive and gram negative bacteria.
Antimicrobial studies were carried out by the agar-cup diffusion test against the test bacteria.
Stock solutions of test substance was freshly prepared by dissolving each chemical in dimethyl sulfoxide (DMSO). The conc. of test chemical used for the study was 12500 mg/l (12.5 mg/ml).
10 different test organisms were used for the study. They include - B. subtilis ATCC 6633, Bacillus idosus B241, B. megat.NRRL 1353895, Bacillus mycoides DSMZ 274, Bacillus cereus ATCC 11778, Acinetob. johnst. ATCC 17909, Staph. aureus NRRL B 313, Sarcina lutea ATCC 9341,Microc. luteus ATCC 9631 and E. coli ATCC 8739, respectively.
The bacterial strains were grown in nutrient agar for 24 h at 37°C.
Suspensions of the test microorganisms were inoculated into sterile melted nutrient agar media and poured into Petri dishes. Six per dish wells, each 8 mm in diameter, were prepared. Fifty microliter of each test sample in dimethylsulfoxide (DMSO) (25 mg/ml) was added to the appropriate well. For pre-diffusion the Petri dishes were placed at 4°C for 2 h. The antimicrobial activity was estimated by the diameter of inhibitory zones (mm) in the agar layer. Control experiments were carried out with the pure solvent.Streptomycin was used as a reference substance for the study.
Based on growth inhibition of test organism, the LOEC value was found to be 12500 mg/l.
In a supporting study on similar substance (CAS no. 708-06-5) [Binnur Μericli Yapici, et. al (2005)]:
1) Toxicity to micro-organisms study was conducted on 14 different bacteria.
Antimicrobial studies were carried out by the agar-disc diffusion method against the test bacteria.
Test solutions were prepared by dissolving the test substance in DMF solvent. The conc. of test chemical used for the study was 200 mg/l (200 ppm).
14 different bacteria were used for study. They include-
1. Corynobacterium xerosisCCM 2824
2. Proteus vulgarisATCC 6897
3. Staphylococcus epidermidisNRRL B-4877 10.0
4. Staphylococcus aureusATCC 6538 Ρ
5. Enterobacter aerogenesATCC 13048
6. Salmonella thyphimuriumCCM 5445
7. Pseudomonas aeruginosaATCC 27853
8. Escherichia coliATCC 11230
9. Escherichia coliATCC 23998
10. Bacillus cereusATCC 7064
11. Bacillus cereusATCC 99
12. Bacillus subtilisATCC 6633
13. Yersininiaspp. and
14. Neisseria canis.
Test microorganisms were obtained from the culture collection of Ege University Faculty of Science, Basic and Industrial Microbiology Department.
Bacterial cultures were suspended in 7-8 ml brain heart infusion broth (Oxoid). The bacteria were incubated at 37 ± 0.1 °C for 24 hours.Prepared bacterial suspensions were inoculated at 10 μl into Muller Hilton agar. Microbial cultures were separated with L bags and all Petri dishes after inoculation were allowed to dry for 15-20 minutes at room temperature. The concentration of test chemical used was 200 ppm in DMF solvent. These chemicals were impregnated into 6 mm diameter discs at 10 μl. All discs were dried at 50°C and placed into the Petri dishes containing the bacteria. Inhibition zone diameters were measured after 24-48 hours using the agar-disc diffusion method.
Based on growth inhibition of test organism, the LOEC value was found to be 200 mg/l.Thus based on this value it can be concluded that the substance can be classified as non- hazardous as per the criteria of CLP regulation.
2) Toxicity to micro-organisms study was conducted on 8 different filamentous fungi.
Antimicrobial studies were carried out by the agar-disc diffusion method against the filamentous fungi.
Test solutions were prepared by dissolving the test substance in DMF solvent. The conc. of test chemical used for the study was 200 mg/l (200 ppm).
8 different filamentous fungi were used for the study. They are-
1. Aspergillus niger
2. A. fumigates,
3. A. versicolor,
4. A. flavus,
5. A. parasiticus,
6. Penicillium granulatum,
7. P. chrysogenum,and
8. P. herque
Test microorganisms were obtained from the culture collection of Ege University Faculty of Science, Basic and Industrial Microbiology Department.
The solutions of OHNA were added to Petridishes to 100 μl. The sterilized medium at 45-50°C was poured into 90 mm diameter Petri dishes. All Petri dishes were allowed to dry for 15- 20 minutes at room temperature. Spore suspensions of filamentous fungi were inoculated onto malt extract agar at 105cfu/ml by plate dilution techniques using Thomas slides. The evaluation of filamentous fungi was carried out by means of reproduction on the medium and reduction of colony numbers at the end of a 7-day period.
As no inhibitory effect on growth of test organisms were observed, the NOEC value was found to be 200 mg/l.Thus based on this value it can be concluded that the substance can be classified as non- hazardous as per the criteria of CLP regulation.
On the basis of the above results, the substance can be classified as non- hazardous as per the criteria of CLP regulation.
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