Androsta-3,5-dien-17-one, 3-(acetyloxy)-, cyclic 17-(2,2-dimethyl-1,3-propanediyl acetal)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

other information

Study period:

Dec 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(9aR,11aS)-9a,11a-dimethyl-1-oxo-1H,2H,3H,3aH,3bH,4H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-7-yl acetate

EC Number:

927-431-5

Cas Number:

1178896-82-6

Molecular formula:

C26 H38 O4

IUPAC Name:

(9aR,11aS)-9a,11a-dimethyl-1-oxo-1H,2H,3H,3aH,3bH,4H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-7-yl acetate

Details on test material:

- Name of test material (as cited in study report): Dienolacetat-ketal (ZK67985)
- Lot/batch No.: 51537013-021

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

biomass

Any other information on results incl. tables

The biomass and growth rate was inhibited (> 10%) at dilutions from 1:5 and saturated solution,

respectively. This was considered to be a compound related effect.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Dienolacetat-ketal was considered to be slightly toxic to the green algae Desmodesmus
subspicatus, since the EC50 was higher than a saturated solution, however the toxicity threshold
is below 1 mg/L .

Executive summary:

The purpose of this study was to detennine the effects of the test compound Dienolacetat-ketal (ZK 67985) on the growth of the green algae Desmodesmus subspicatus. ZK 67985 is an intennediate of the synthesis of drospirenone. The study was conducted in agreement with the test guideline OECD no. 201.

The test substance was incubated in an aqueous solution including nutrients with an algae population of Desmodesmus subspicatus for a test duration of approximately 72 hours in an electronically controlled dosing and incubation apparatus. As a parameter for the growth of the algae population, the fluorescence of the algal cells was measured with a fluorescence-photometer.

Dienolacetat-ketal had a concentration-depended inhibitory effect on the growth of Desmodesmus subspicatus. However, the inhibition was below 50%, therefore the EbC50 (biomass) and the ErC50 (growth rate) was higher than a saturated solution (>1 mg/L).