Benzenebutanoic acid, 4-[(4-methoxybenzoyl)amino]-.beta.-methyl-3-nitro-.gamma.-oxo-, methyl ester

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 January 1997 - 07 February 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: compliant to GLP and testing guideline; adequate coherence between data, comments and conclusions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

426-290-2

EC Name:

-

Cas Number:

74149-72-7

Molecular formula:

C20H20N2O7

IUPAC Name:

methyl 4-[4-(4-methoxybenzamido)-3-nitrophenyl]-3-methyl-4-oxobutanoate

Details on test material:

- Name of test material (as cited in study report): UD CG 115 BS-GAMMA NAP
- Substance type: monoconstituent
- Physical state: yellow fine powder
- Analytical purity: 99.8%
- Lot/batch No.: 1/94
- Expiration date of the lot/batch: 01 October 1998
- Stability under test conditions: stable
- Storage conditions of test material: at room temperature, protected from light.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

activated sludge freshly obtained from municipal sewage treatment plant

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0.6

Sampling time:

2 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

0.9

Sampling time:

7 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

0.9

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

1.1

Sampling time:

27 d

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

1.2

Sampling time:

29 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Not easily biodegradable, including when ignoring 10-day window.
As the substance is poorly water soluble but not insoluble (0.41 mg/L), is practically non biodegradable, and has a log Kow > 3 (3.5), it should be
labelled with risk phrase R53.

Executive summary:

Not easily biodegradable, including when ignoring 10-day window, due to only 1% degradation in 29 days. Almost non degradable.