Registration Dossier
Registration Dossier
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EC number: 941-122-2 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 2,6-dimethylpyridinium chloride and 3-methylpyridinium chloride and 4-methylpyridinium chloride
- EC Number:
- 941-122-2
- Molecular formula:
- not yet assigned
- IUPAC Name:
- Reaction mass of 2,6-dimethylpyridinium chloride and 3-methylpyridinium chloride and 4-methylpyridinium chloride
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Suspended matter: 10ml/L
- Colony forming units (CFU): 1.35 x 10^9 CFU/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 29 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: Actual: 21.5 – 21.7 °C
- pH: Start: 7.49; End: 1) 7.84, 2) 7.82; [1), 2) = First, second replicate]
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: 500 mL glass vessels
- Number of culture flasks/concentration:
•two for the test item (P1, P2)
•one for the functional control (R1)
•one for the toxicity control (T1)
•two for the inoculum control (C1, C2)
- Measuring equipment: OxiTop OC110 controller, WTW ; OxiTop measuring heads, WTW
SAMPLING
- Sampling frequency: The oxygen consumption was determined in the incubation vessels by the OxiTop® measuring system at 360 measuring points (every 112 minutes) during the 28 d incubation period.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 45
- Sampling time:
- 28 d
- Remarks on result:
- other: Replicate 1
- Parameter:
- % degradation (O2 consumption)
- Value:
- 42
- Sampling time:
- 28 d
- Remarks on result:
- other: Replicate 2
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance: 82% after 7 days, 89% after 14 days, 97% after 21 days, 96% after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The test item is classified as not readily biodegradable in the 10-day-window and within 28 days.
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