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EC number: 690-526-2 | CAS number: 38632-47-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Additional information
Isotan is an organic salt of strong base NaOH and weak acid causing a pH-shift in water. The tolerance of water organisms towards pH is diverse. Recommended pH values for test species listed in OECD guidelines are between 6 and 9.
Acute toxicity to fish (Brachydanio rerio) was determined in accordance with the Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992). Groups of seven fish of the recommended size were exposed to a range of concentrations, nominally 9.4; 20.7; 45.5 and 100 mg/L of Isotan, dissolved in water of pH value 6.0 under semi-static conditions. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects. After 96 h LC 50-values of 60.01 mg/l for the active ingredient and 65.86 mg/l for the drying residue (geometric mean measured concentrations) were measured.
Concerning the toxicity towards aquatic invertebrates, an acute toxicity test to Daphnia magna was taken into account to draw a conclusion on toxicity towards aquatic invertebrates for Isotan. The study was performed in accordance with the Council Regulation (EC) No 440/2008, Method C.2 'Acute toxicity for Daphnia' (2008) which is in most parts equivalent to the OECD Guideline 202 (Daphnia sp. Acute Immobilization Test). Daphnia magna was exposed under semi-static conditions to a range of concentrations of the test item (pH 6.0-7.0). After 48h EC 50-values of 24.7 mg/l for the active ingredient and 27.1 mg/l for the drying residue were (geometric mean measured concentrations) were measured.
In a test, the fresh water alga Scenedesmus subspicatus was exposed to Isotan for 72 hours under static conditions (pH 6.1-7.2). The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Algae and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test' (2006). Exponentially growing algal cells were exposed for a period of 72 hours to a range of concentrations, nominally 0.32, 1.0, 3.2, 10 and 32 mg/L of the test item dissolved in water. The cell densities were measured at 24 hour intervals. The study data were analyzed on a 48 h basis. As cell numbers increased well above 16-fold over 48 h, and all other validity criteria are met, this is an option explicitly covered by OECD TG 201 (2006) and EU method C.3 (2009). After 48 h EC 50-values of > 100 mg/l for the active ingredient and the drying residue (geometric mean measured concentrations) were measured.
In order to test the toxicity to microorganism of the substance, activated sludge from predominantly domestic sewage plant was exposed to the test solution of five nominal concentrations of the substance (100, 180, 320, 560, 1000 mg/l), blank control solution and solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and respiration rates were calculated. 49.0 % respiration inhibition was observed at the highest concentration of test substance as water solution. Analysis of the respiration rates after 3 hours (by probit analysis) gave an EC 50 value of 978.2 mg/l and an EC 10-value of 85.0 mg/l. As the content of the active ingredient in the water solution is 36.5% the effect concentrations can be calculated for the pure and solid substance as EC50= 357.0 mg/L and EC10= 31.03 mg/L. The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.11 ”Activated sludge respiration inhibition” (2008). This test method is equal to OECD Guideline 209 (1984).
All toxicity studies mentioned above are evaluated as acceptable and satisfy the guideline requirements for the toxicity studies on acute toxicity to aquatic organisms.
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