3-(3-tert-butyl-4-hydroxyphenyl)propionic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Mar. 22, 1988 to May 2, 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented, according to accepted guideline but only for 24 hours, with GLP-like Quality Assurance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

test duration 24 hours; Highest vechicle concentration: 950 mg/l DMF

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-(3-tert-butyl-4-hydroxyphenyl)propionic acid

EC Number:

403-920-4

EC Name:

3-(3-tert-butyl-4-hydroxyphenyl)propionic acid

Cas Number:

107551-67-7

Molecular formula:

C13H18O3

IUPAC Name:

3-(3-tert-butyl-4-hydroxyphenyl)propanoic acid

Details on test material:

- Physical state: beige crystalls
- Analytical purity: commercial grade
- Lot/batch No.: EN.116905.72
- Expiration date of the lot/batch: 11/92
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 10, 18, 32, 58 and 100 mg/l
- Sampling method: 50 ml (or the whole volume if less than 50 ml are available) are taken after intensively stirring and filled into a 100 ml glass bottle.
Two samples must be taken.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
1.0 g test substance was dissolved in and made up to 10 ml with DMF (dimethyl formamide). This solution was diluted to 100 mg/l with water.
- Controls: negative control, solvent control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethyl formamide (DMF)
- Concentration of vehicle in test medium (stock solution and final test solution): 950 mg DMF/l maximum

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: DAPHNIA MAGNA STRAUS 1820.
- Source: CIBA-GEIGY Ltd., Facilities, Basle.
- Age at study initiation (mean and range, SD): young daphnia (0 - 24 h old).
- Length at study initiation (length definition, mean, range and SD): < 300 micrometer.
- Method of breeding: Parthenogenetic reproduction.
Feed: Suspension of green algae supplenented by trout chow and/or powder milk and/or yeast; in such quantities that food is consuned after 24 hours
- Feeding during test: None during the test.

- Pretreatment:
24 hours before the start of the test reproductive daphnia ars separated fron the young by sieving through a 300 micrometer sieve. This operation is repeated immediately before the start and the young daphnia (0 - 24 h old) are retained for the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

Not applicable.

Test conditions

Hardness:

240 mg CaC03/L.

Test temperature:

20 ± 1 ° C

pH:

6.9-7.8

Dissolved oxygen:

Values not determined.

Salinity:

freshwater

Nominal and measured concentrations:

Refer to table 1.

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers covered with watch glasses (100 ml solution)
- Aeration: None during the test; the water was aerated with clean air for at least 24 h before use.
- No. of organisms per vessel: 20 daphnia/conc. and control. (4 replicates of 5 daphnia)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water produced by dissolving :
65 mg NaHCO3
274 mg CaCl2 ; 2 H2O
123 mg MgSO4 ; 7 H2O
6 mg KCl
in 1000 ml bidistilled water.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours fluorescent light daily.
- Light intensity: approx. 2000 lux.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilization, pH and temperature

TEST CONCENTRATIONS
- Spacing factor for test concentrations: Not exceeding 2.2.

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: None

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: None

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: None

Details on results:

Refer to Table 2.

Results with reference substance (positive control):

Controls :
Immobilization blank (water) 0 %.
Immobilization vehicle (DMF) 0 %.

Reported statistics and error estimates:

None.

Any other information on results incl. tables

Table 2.Immobilization

Concentrations nominal (mg/L)	Immobilization after 24 hours
	1	2	3	4	total	%
Blank	0	0	0	0	0	0
Vehicle	0	0	0	0	0	0
10	0	0	0	0	0	0
18	0	0	0	0	0	0
32	0	0	0	0	0	0
58	0	0	0	0	0	0
100	0	0	0	0	0	0