potassium {[(2Z)-4-ethoxy-4-oxobut-2-en-2-yl]amino}acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

32085-79-3

EC Number:

608-700-3

Cas Number:

32085-79-3

IUPAC Name:

32085-79-3

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The analytical verification of the test item concentrations was carried out after 0 and 48 hours. For stability control a series of the concentrations
tested, was made up in the same way as the concentrations for biological part, but in separate vessels without test organisms.

Test solutions

Vehicle:

yes

Details on test solutions:

Stock solution: For the preparation of a stock solution, 10 g/L of the test item was equilibrated in completely desalted water. The resulting solution
was used as stock solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Name: Daphnia magna Straus, clone 5
Origin: The water flea Daphnia magna Strauss used is that of clone 5. Originally, the animals were received 1991 from Dr. N. Caspers (Bayer AG,
Leverkusen, Germany); subsequently they were bred in house. The operating steps necessary to breed the animals are given in a Standard
OperatingProcedure.
Culture conditions: For breeding, the females are maintained in M4 medium in 1 L beakers. The daily amount of food, comprising Desmodesmus
subspicatus, is such that the amount added is consumed by the animals. A reddish dark-brown coloration of the animals indicates that they are
healthy. At two to three day intervals, the exuviae are syphoned off and the water is changed. ln the course of this, the offspring is removed from thebreeding vessels. At regular intervals of approximately 4 weeks, offspring is isolated from the vessels. They serve as a basis for further breeding.
The culture conditions ensure parthenogenetic reproduction.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

14°dH = 250 mg CaCO3/L

Test temperature:

20.0-20.3°C

pH:

7.7-9.5

Dissolved oxygen:

7.5-9.1 mg/L

Nominal and measured concentrations:

nominal 250, 350, 500, 700and 1000 mg/L

Details on test conditions:

Test vessels: Round-bottom test tubes calibrated to 10 ml
Experimental set-up: 5 test item concentrations + 1 control, 20 organisms in 4 parallel sets of 5 each
Density of occupation of animals: ca. 2 ml per test organism
Light/dark cycle: dark
Temperature: 20.0- 20.3°C (mean: 20.2°C)
Aeration: none
Feeding: none
Solubilizer: none

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The highest concentration tested in this experiment at which after 48 h exposure no immobilisation (<=10%) occurred, was 1000 mg/L.
The lowest concentration tested in this experiment at which after 48 h exposure 100 % immobilisation occurred, was >1000 mg/L.

Results with reference substance (positive control):

Daphnia magna. lnfluence of potassium dichromate on the swimming behaviour after 24 h exposure.
concentration (mg/ L) % of immobilised Daphnia
1.0 20
2.0 100
The results demonstrate that the sensitivity of the individuals lies in the normal range.