8-Chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Cas Number:

353258-35-2

Molecular formula:

C9H4ClF3N2O2

IUPAC Name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Specific details on test material used for the study:

Test substance: IN-QEK31-011
Batch No.: SG0312574
Purity: 98.2%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Remarks:

water

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

250 mg/L as CaCO3

Test temperature:

13 - 15°C

pH:

7.2 - 7.7

Dissolved oxygen:

60 to 101 % of the air saturation value

Salinity:

0.8 mmol/L

Nominal and measured concentrations:

Nominal: 10, 4.5, 2.1, 0.94 and 0.43 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: sublethal effects

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: sublethal effects

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

96-hr LC50 (rainbow trout): >10 mg/L (highest concentration tested)
96-hr NOEC (rainbow trout): 10 mg/L
96-hr LOEC (rainbow trout): >10 mg/L

Executive summary:

The purpose of this study was to evaluate the acute toxicity of the test item to fish in accordance with OECD Guideline 203. For this purpose, juvenile Rainbow Trout (Oncorhynchus mykiss) were exposed in a static test to aqueous test media containing the test item at various concentrations under defined conditions. The recorded effects were the mortality and sublethal effects on the fish.

The acute toxicity of the test item on Rainbow Trout (Oncorhynchus mykiss) was determined in ISO Medium (prepared according to OECD guideline 203). Five nominal test concentrations of 10, 4.5, 2.1, 0.94 and 0.43 mg/L and a test water control with 7 test animals each were incubated in a controlled environment for 96 hours at 13 to 15°C. Sublethal effects and mortality were determined and recorded at test start and after 2, 24, 48, 72 and 96 hours of test duration.

No mortality or sublethal effects were observed in the control and at the five test item concentrations during the whole test duration.

The toxic effect of the test item to Rainbow Trout (Onchorhynchus mykiss) was determined in a static dose-response test. All fish survived until the end of the experiment and showed no sublethal effects during the exposure time, except of two fish with dark coloration after 24 hours in the highest test concentration (10 mg/L). After 48 hours this effect could not be seen anymore and therefore does not appear to be a substance related effect.

The 96 hour LC50 value was therefore calculated to be > 10 mg/L. The 96-hour NOEC was 10 mg/L and the 96-hour LOEC was > 10 mg/L.