2-propen-1-amine, N-2-propen-1-yl-, hydrochloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 March - 15 April 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

The temperature of the tests remained within 20 °C 2 °C but a few vessels did not remain within a constant 1 °C. This has not thought to have had a significant impact on the overall test result as the control vessels met the validity criteria.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

66% purity
Aqueous solution

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution Series

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation: The ephippia were incubated no more than 96h prior to test commencement, therefore the neonates were less than 24h old at test commencement
- Source: The test organisms were sourced from ephippia supplied by Cheshire Eco Solutions Ltd, from MicroBioTests
- Feeding during test : The animals were fed Pseudokirchneriella subcapitata during incubation and not fed during the test.


ACCLIMATION
- Acclimation period: The ephippia were incubated no more than 96h prior to test commencement
- Type of food: Pseudokirchneriella subcapitata

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Observations were recorded at 24 hours and 48 hours of exposure to test substance

Hardness:

Not specified

Test temperature:

20 °C 2 °C. Constant within 1 °C

pH:

6-9

Dissolved oxygen:

>3 mg/l

Salinity:

Not specified

Conductivity:

Not specified

Nominal and measured concentrations:

10, 32, 100, 320, 1000, 3200,10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass crystallising dishes
- Material, size, headspace, fill volume: 100 ml capacity, borosilicate
- Volume of solution: 50ml
- Aeration: covered with soda glass watch covers
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2 vessels per concentration
- No. of vessels per control (replicates): 2 vessels per concentration
- No. of vessels per vehicle control (replicates): 2 vessels per concentration

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO reconstituted water (as specified in OECD 202)
- Intervals of water quality measurement: Carried out at each concentration at 0h and 48h

EFFECT PARAMETERS MEASURED: Immobilisation

OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark

RANGE-FINDING STUDY
- Test concentrations: 1000, 100, 10 and 1 mg/l
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 304 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

ca. 1 730 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none reported
Observations on body length and weight: None reported
- Mortality of control: 5%
- Abnormal responses: None reported

Results with reference substance (positive control):

- Results with reference substance valid? YES
- Relevant effect levels: yes
- Limit test: no
- Dose-response test: yes
- EC50 24h: 0.6 - 2.1 mg/L

Reported statistics and error estimates:

Results for both range-finding and definitive tests were calculated by Linear Interpolation within the CETIS suite of statistical analysis.

Nominal Concentration (mg/l)	Number immobile at 24h		Number immobile at test termination (48h)		Total Exposed
	Replicate A	Replicate B	Replicate A	Replicate B
10	0	0	0	1	20
32	0	0	1	0	20
100	1	0	2	2	20
320	2	1	5	6	20
1000	3	2	9	9	20
3200	10	9	10	9	19
10000	11	10	11	10	21

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 was 304 mg/L and the 48-hour NOEC 32 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°
1272/2008.

Executive summary:

The short-term toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater crustacean Daphnia magna was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.

A range-finding test was conducted at concentrations of 1000, 100, 10 and 1 mg/l, plus four control vessels to determine the concentrations to be used during the definitive test.

In the definitive test, concentrations of 10, 32, 100, 320, 1000, 3200,10000 mg/L, plus four control vessels were used.

The 48-hour EC50 was 304 mg/L and the 48-hour NOEC 32 mg/L. 

Description of key information

The short-term toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater crustacean Daphnia magna was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.

A range-finding test was conducted at concentrations of 1000, 100, 10 and 1 mg/l, plus four control vessels to determine the concentrations to be used during the definitive test.

In the definitive test, concentrations of 10, 32, 100, 320, 1000, 3200,10000 mg/L, plus four control vessels were used.

The 48-hour EC50 was 304 mg/L and the 48-hour NOEC 32 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

304 mg/L