Triisopropylsilyl acrylate

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 February 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

(Date of inspection: 02 December 2002 Date of signature: 13 February 2003)

Type of method:

HPLC estimation method

Media:

other: methanol

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): TIPX
- Substance type: Colourless liquid.
- Physical state: Liquid.
- Lot/batch No.: 042028.
- Storage condition of test material: Approximately 4ºC in the dark.

Radiolabelling:

no

Study design

Test temperature:

(Column temperature for HPLC analysis) 25°C.

HPLC method

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: HPLC, Agilent Technologies 1100, incorporating autosampler and workstation.
- Column: Phenosphere NEXT CN, 5µ (250 x 4.6 mm id).
- Flow rate: 1 ml/min
- Injection volume: 10 µl

MOBILE PHASES
- Type: methanol: water (55:45 v/v)
- pH: 5.9

DETERMINATION OF DEAD TIME
The dead time was determined by measuring the retention time of formamide (purity, quoted by supplier, ≥99.5%, 160 mg/l solution in methanol).
- Dead time and reference standards: 210 nm
- Sample: 210 nm

REFERENCE SUBSTANCES
- Identity: acetanilide, phenol, atrazine, isoproturon, triadimenol, linuron, naphthalene, endosulfan-diol, fenthion, phenanthrene, diclofop-methyl and DDT. See concentrations of each reference substance in Table 1.

EVALUATION
The capacity factor was calculated using the following formula and the log10Koc value determined with reference to the calibration curve;
k = (tr - t0) / t0
where:
k = capacity factor
tr = retention time (min)
t0 = dead time (min)

Results and discussion

Adsorption coefficient

Type:

log Koc

Value:

4.22

Temp.:

25 °C

Remarks on result:

other: Temperature as column temperature.

Results: HPLC method

Details on results (HPLC method):

See results of absorption coefficient of the sample in Table 2.

- Retention times of reference substances used for calibration: See Table 3.

- Details of fitted regression line (log k' vs. log Koc): See the Figure, as attached.

- Average retention data for test substance: 12.176 mins.

Any other information on results incl. tables

Table 1 Reference substances

Standard	Purity (%), quoted by supplier	Concentration (mg/l)
Acetanilide	~99	128
Phenol	99.99	166
Atrazine	99.2	133
Isoproturon	99	116
Triadimenol	98.5	127
Linuron	99	112
Naphthalene	³99.0	116
Endosulfan-diol	99.9	116
Fenthion	96	153
Phenanthrene	³97	105
Diclofop-methyl	99.3	111
DDT	98	147

Table 2 Adsorption Coefficient of the Sample

Injection	Retention Time (mins)	Capacity Factor (k)	log10k	log10Koc
1	12.178	2.49	0.396	4.22
2	12.174	2.49	0.396	4.22

Table 3 Calibration Results

Standard	Retention Time (mins)		Mean Retention Time (mins)	Capacity Factor (k)	Log10k	Log10Koc
	Injection 1	Injection 2
Formamide	3.49	3.493	3.492	-	-	-
Acetanilide	3.834	3.836	3.835	9.84 x 10^-2	-1.01	1.25
Phenol	3.76	3.76	3.76	7.69 x 10^-2	-1.11	1.32
Atrazine	4.247	4.245	4.246	0.216	-0.665	1.81
Isoproturon	4.558	4.556	4.557	0.305	-0.515	1.86
Triadimenol	5.185	5.186	5.186	0.485	-0.314	2.4
Linuron	5.324	5.326	5.325	0.525	-0.28	2.59
Naphthalene	5.809	5.805	5.807	0.663	-0.178	2.75
Endosulfan-diol	6.15	6.142	6.146	0.76	-0.119	3.02
Fenthion	7.971	7.972	7.972	1.28	0.108	3.31
Phenanthrene	8.684	8.694	8.689	1.49	0.173	4.09
Diclofop-methyl	12.492	12.487	12.49	2.58	0.411	4.2
DDT	35.359	35.337	35.348	9.12	0.96	5.63

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient (Koc) of the test material has been determined to be 1.68E+04, log10 Koc 4.22.

Executive summary:

Adsorption Coefficient has been determined to be 1.68 x 10⁴, log₁₀K_oc 4.22, using the HPLC screening method, Method C19 of Commission Directive 2001/59/EC.