Reaction mass of trimethyl-3-[(1-oxooctadecyl)amino]propylammonium methyl sulphate, Propane-1,2-diol and Trimethyl-3-[(1-oxohexadecyl)amino]propylammonium methyl sulphate

Endpoint summary

Description of key information

LC50 (96h) for fish = between 0.1 and 1 mg/l.

EC50(48h) for daphnia < 1 mg/l, based on mobility.

EC50(72h) for alga < 1 mg/l, based on growth rate.

EC50(3h) for microorganisms > 10 mg/l, based on respiration inhibition.

Additional information

Short-term toxicity to fish was tested according to OECD guideline 201.

A preliminary test on series of three fishes indicated that no mortality was expected for test item concentrations of 0.01 and 0.1 mg/l after a period of 4 days. Mortality was however observed for the three individuals at test item concentrations of 1, 10 and 100 mg/l.

A definitive study was carried out on two series of 10 individuals with a concentration of 0.1 mg/l of test item for a period of 4 days. Two repetitions were performed. No mortality was observed.

Based on these results, the 96-hour LC50 value for acute toxicity to fish of the test item was assumed to be between 0.1 and 1 mg/l.

Short-term toxicity to aquatic invertebrates was tested according to OECD guideline 202. Based on immobilisation, the 48 -hour EC50 value was < 1 mg/l.

Toxicity to aquatic algae was tested according to OECD guideline 201. Based on growth rate and yield, the 72 -hour EC50 value was < 1 mg/l.

Respiration inhibition on activated sludge was studied according to OECD guideline 209. The activated sludge was tested at various concentrations of test substance for 3 consecutive hours.

A preliminary study was carried out with test substance concentrations of 1, 10, 100 and 1000 mg/l.

Based on the results obtained in this preliminary test, it was decided to make new measurements on 10 mg/l replicates.

No significant respiration inhibition was found in the 10 mg/l replicates.

The critical micelles concentration was determined experimentally to be 140 mg/l, thus far above the concentrations tested in aquatic toxicity studies.

Available studies on fish, aquatic invertebrates and algae are indicative of a high toxicity of test substance to aquatic species.

According to the CLP Regulation (EC 1272/2008), short-term (acute) aquatic hazard category Acute 1 applies if:

96 hr LC50 (for fish) ≤ 1 mg/l and/or 48 hr EC50 (for crustacea) ≤ 1 mg/l and/or 72 or 96 hr ErC50 (for algae or other aquatic plants) ≤ 1 mg/l.

Consequently, test substance is classified as Acute 1 (H400: Very toxic to aquatic life).

According to the CLP Regulation (EC 1272/2008), a classification as category Chronic 1 for long-term (chronic) aquatic hazard applies for substances for which adequate chronic toxicity data are not available if: 96 hr LC50 (for fish) ≤ 1 mg/l and/or 48 hr EC50 (for crustacea) ≤ 1 mg/l and/or 72 or 96 hr ErC50 (for algae or other aquatic plants) ≤ 1 mg/l and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log Kow ≥ 4).

No chronic toxicity data on the substance is available; however, the substance is not ready biodegradable and, based on acute toxicity data, it is classified as Chronic 1 (H410: Very toxic to aquatic life with long-lasting effects).