2-Butanone, 1,3,4-trihydroxy-

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

EEC Directive 92/69, Publication No. L383 Part C-3 adopted December, 1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

OECD guideline No. 201 , Adopted June 7, 1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)

Version / remarks:

ISO Standard 8692, First edition, 15 November 1989

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

NA

Analytical monitoring:

yes

Details on sampling:

Frequency: at t=24 h and t= 72h
Volume: 10 mL

Vehicle:

yes

Details on test solutions:

Preparation of test solutions started with a stock solution of 100 mg/l. No special treatment other than a 10-minute period of magnetic stirring was necessary to dissolve the test substance in the test medium. The lower test concentrations were prepared by subsequent dilutions of the stock in test medium. The final test solutions were all clear and colourless.
After preparation, volumes of 50 ml were added to each replicate of the respective test concentration. Subsequently, adequate volumes of an algal suspension were added to each replicate providing a cell density of 10^4 cells/ml.

Test organisms (species):

Selenastrum sp.

Details on test organisms:

Selenastrum capricornutum, strain: NIVA CHL 1

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

NA

Hardness:

Hardness (Ca+Mg) 0.24 mmol/l (24 mg CaCO3)

Test temperature:

21-25°C, constant within 2°C.

pH:

pH: 8.3 ± 0.2

Dissolved oxygen:

NA

Salinity:

NA

Conductivity:

NA

Nominal and measured concentrations:

The concentrations measured in samples taken from 100 mg/l were in agreement with nominal at the start and after 24 hours of exposure. At the end of the test period the measured concentration had decreased to 80 mg/l, i.e. by 18% relative to the initial start concentration. The measured concentration in samples taken from 100 mg/l without algae showed that the concentration remained stable during the test period. Hence, the slight decrease in the solution with algae was expected to be due to absorption to algal cells. All results will be based on nominal concentrations.

Details on test conditions:

NA

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

Algae were exposed for a period of 72 hours to K2Cr207 concentrations of 0.18, 0.32, 0.56, 1.0, 1.8 and 3.2 mg/l and to a blank-control. The initial cell density was 1.0 x 10^4 cells/ml. Under the conditions of the reference study with Selenastrum capricornutum, potassium dichromate inhibited cell growth of this fresh water algae species at nominal concentrations of 0.56 mg/l and higher and reduced growth rate at 1.0 mg/l and higher.

No remarks

Validity criteria fulfilled:

yes

Conclusions:

No inhibition of cell growth or reduction of growth rate was recorded at any of the concentrations of (S)-1,3,4-trihydroxybutan-2-one tested.
Hence, the EC50 for both cell growth inhibition and growth rate reduction was above 100 mg/l, the regulatory limit concentration.

Executive summary:

No inhibition of cell growth or reduction of growth rate was recorded at any of the concentrations of (S)-1,3,4-trihydroxybutan-2-one tested.

Hence, the EC50 for both cell growth inhibition and growth rate reduction was above 100 mg/l, the regulatory limit concentration.

Description of key information

No inhibition of cell growth or reduction of growth rate was recorded at any of the concentrations of (S)-1,3,4-trihydroxybutan-2-one tested.
Hence, the EC50 for both cell growth inhibition and growth rate reduction was above 100 mg/l, the regulatory limit concentration.

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L