3-cyclohexylaminopropylamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978-09-13 to 1978-09-27

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

Exposure duration 60 minutes, higher limit dose

GLP compliance:

no

Test type:

traditional method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-cyclohexylaminopropylamine

EC Number:

222-001-7

EC Name:

3-cyclohexylaminopropylamine

Cas Number:

3312-60-5

Molecular formula:

C9H20N2

IUPAC Name:

N1-cyclohexylpropane-1,3-diamine

Test material form:

liquid

Details on test material:

3-Cyclohexylaminopropylamine from Performance Chemicals, lot number: 68-219-BC

Test animals

Species:

rat

Strain:

other: Sprague-Dawley CD rats derived by Charles River

Sex:

male/female

Details on test animals or test system and environmental conditions:

Test animals purchased from Charles River Breeding Laboratories. Rats were individually housed in metal hanging cages of appropriate size prior to exposure and in plastic crispers with Sanicel bedding during the observation period. All animals were maintained in isolated temperature and humidity controlled animal rooms with filtered air supply and cycled lighting (12 hours of light daily). Purina Laboratory Chow and tested tap water were available ad libitum, except during the exposure time. Rats were acclimated for at least one week prior to initiation of the study.
Rats were selected from stock animals on the basis of body weight and general good health and allocated 5 males and 5 females per dose group. Body weight of young adult rats was between 194 - 238 g.

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

clean air

Mass median aerodynamic diameter (MMAD):

>= 0.5 - <= 3 µm

Remark on MMAD/GSD:

not specified

Details on inhalation exposure:

The test material was metered from a 3 L reservoir into the continous feed nebulizer chamber of a Devilbiss Ultrasonic Nebulizer (Model 6582)capable of delivering liquid particles in a size range of 0.5 to 3.0 microns. A steam of filtered air wa passed through the nebulizer to produce concentrated aerosol which was introduced into the chamber at the top. The average nominal exposure concentration was calculated by dividing the total weight loss of the test material by the total volume of air used for aerosolination and dilution over the period of operation. The test atmosphere was exhausted at the bottom through an activated charcoal filter to archieved dynamic flow through the chamber.

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

ca. 60 min

Remarks on duration:

After single exposure for 60 minutes the rats were observed for the following 14 days

Concentrations:

7.5 mg/L

No. of animals per sex per dose:

5 groups of 5 males and 5 females (50 animals)

Control animals:

yes

Remarks:

exposed to air only

Details on study design:

Each dose group was placed in a 400 L stainles steal and glass inhalation chamber and caged individually in stainless steal wire mesh cages ( 5 x 5 x 18 cm).

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 7.5 mg/L air

Exp. duration:

60 min

Remarks on result:

not determinable due to absence of adverse toxic effects

Mortality:

no effects during the 14 day observation period

Clinical signs:

other: no signs of toxicity observed

Body weight:

no differences between control and dosed groups and no differences between males and females

Gross pathology:

Gross necropsy of all rats after 14 days and examination of the major organs did not reveal any remarkable changes that were attributed to treatment.

Applicant's summary and conclusion

Conclusions:

Under the conditions of this test, the LC50 could not be determined for the test compound. Based on the results, the LC50 would be greater than 7.5 mg/L.

Executive summary:

After single whole body exposure of young CD rats to the test compound for 60 minutes, no toxicological effects could be seen during the 14 days observation period after the exposure.