(S)-α-hydroxy-3-phenoxy-benzeneacetonitrile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

441-070-6

EC Name:

-

Cas Number:

61826-76-4

Molecular formula:

C14H11NO2

IUPAC Name:

(2S)-2-hydroxy-2-(3-phenoxyphenyl)acetonitrile

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples of test media for determination of the actual concentrations of the test item and the degradation product were taken before settin-in of the fish and after ca. 4 and 96 hours.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

< 250 mg CaCO3/L

Nominal and measured concentrations:

Nominal concentrations: 0, 0.5, 0.9, 1.6, 2.8 and 5.0 mg/L

Details on test conditions:

7 fish each were exposed to five concentrations of the test substance (0.5, 0.9, 1.6, 2.8 and 5.0 mg/L) in dilution water, obtained by mixing local tap water with deionized water to achieve the appropriate water hardness as required by the guideline. Additionally, 7 fish were used for one control group. The following investigations were conducted during the study:
- mortality, observations in life
- total hardness of the control water
- pH in the control and test medium at the start and at 24,48,72 and 96 hours
- temperature in the control and test medium at the start and after ca. 24, 48, 72 and 96 hours.
- dissolved oxygen concentration in the control and test media at the start and after ca. 24, 48, 72 and 96 hours
- the test substance was determined at the start and after 4 hours and the degradation product was determined at the start and after 4 and 96 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

1.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

1.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Temperature was stable through the test period. Dissolved oxygen concentration was always higher than 84% of the air saturation value at the appropriate temperature. The pH value of the test substance solution did not change more than one unit within the test period.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Analytical measurements:
The test substance hydrolyses rapidly. After 4 hours the test  substance concentration was already below the detection limit of 0.2 mg/L. The concentrations of the hydrolysis product were also determined and were stable between 4 hours after begin of the exposure and the end of the test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A static acute toxicity test with Danio rerio, applying a nominal concentration of up to 5.0 mg/L test item was conducted according to EU method C.1. The following lethal concentrations were determined after treatment with the test item:
- 96 h LC50: 1.4 mg/L (95% confidence limit (upper 1.7, lower 1.1)
- 96 h LC0: 0.9 mg/L.

Executive summary:

A static acute toxicity test with Danio rerio, applying a nominal concentration of up to 5.0 mg/L test item, was conducted according to EU method C.1. The following lethal concentrations were determined after treatment with the test item:

- 96 h LC50: 1.4 mg/L (95% confidence limit (upper 1.7, lower 1.1)

- 96 h LC0: 0.9 mg/L.