Reaction mass of 5-[(Disubstituted-dihydrocarbopolycyclyl)diazenyl]-6-hydroxy-4-methyl-2-substituted-1-propyl-1,2-dihydropyridine-3-carbonitrile and 1-Butyl-5-[(disubstituted-dihydrocarbopolycyclyl)diazenyl]-6-hydroxy-4-methyl-2-substituted-1,2-dihydropyridine-3-carbonitrile

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reason / purpose for cross-reference:

read-across source

Conductivity:

Not measured

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No acute toxic effects of exposure were observed throughout the test.

No remarkable observations were made concerning the apperance of the test medium. The test medium was a clear solution throughtout the whole test duration.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

Not applicable.

None

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of FAT 41039/A, structural analogue of FAT 41048, to the freshwater invertebrate Daphnia magna was investigated and gave a 48-Hour EC50 value of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.

Executive summary:

The acute toxicity of FAT 41039/A, structural analogue of FAT 41048, to Daphnia magna was determined in a 48 hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992) and the OECD Guideline for Testing of Chemcials No. 202 (2004).

In order to assess the toxicity of the test item FAT 41039/A containing different components to Daphnia magna, a water accommodated fraction (WAF) was prepared. The method is based on the Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures (2000).

A limit test was performed in accordance the test guidelines to demonstrate that WAFs of the test item has no toxic effect on the test daphnids up the loading rate of 100 mg/L.

Before to the start of the test and prior to each test medium renewal, the test medium was prepared as follows: A dispersion of the test item with the loading rate of 100 mg/L was prepared using ultrasonic treatment and intense stirring. The dispersion was stirred at room temperature in the dark over 96 hours to dissolve a maximum amount of the different components of the test item in the test water. After the stirring period, the dispersion was filtered (0.45 µm membrane filter) and the filtrate was tested on the test fish as WAF. Additionally, a control was tested in parallel.

The analytically measured test item concentration in the WAF with the loading rate of 100 mg/L was below the limit of quantification of the analytical method of 0.3 µg/L at the start and the end of the test. The biological results were based on the loading rate of the WAF of 100 mg/L.

In the control and at the loading rate of 100 mg/L, no mortality or other visible abnormalities were determined during the test period of 96 hours.

The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours) and the 48-hour EC0 of FAT 41039/A to Daphnia magna were determined to be the loading rate of at least 100 mg/L. The 48-hour NOEC and the 48-hour EC0 might even be higher but loading rates above 100 mg/L were not tested in accordance with the test guidelines. The 48-hour EC50 and the 48-hour EC100 were clearly higher than the loading rate of 100 mg/L. These values could not be quantified, since the test item had no toxic effects on the daphnids up to the loading rate of 100 mg/L.

In conclusion, the test item FAT 41039/A had no acute toxic effects on Daphnia magna up to the loading rate of 100 mg/L under the present conditions of the test.

Description of key information

The acute toxicity of FAT 41039/A, structural analogue of FAT 41048, to the freshwater invertebrate Daphnia magna was investigated and gave a 48-Hour EC50 value of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

No study on toxicity potential of FAT 41048 to aquatic invertebrates is available. However, the acute toxicity of FAT 41039/A, structural analogue of FAT 41048, to Daphnia magna was determined in a 48 hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992) and the OECD Guideline for Testing of Chemcials No. 202 (2004). In order to assess the toxicity of the test item FAT 41039/A containing different components to Daphnia magna, a water accommodated fraction (WAF) was prepared. The method is based on the Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures (2000).

A limit test was performed in accordance the test guidelines to demonstrate that WAFs of the test item has no toxic effect on the test daphnids up the loading rate of 100 mg/L.

The test item FAT 41039/A had no toxic effect on Daphnia magna up to the loading rate of 100 mg/L. The 48 hour EC50 and NOEC values were determined to be greater than 100 mg/L, respectively.