Naphthalenesulfonic acid, butyl-, Me derivs., sodium salts

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

12 January to 10 March, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The data is read across from a similar substance in a category approach. See chapter 13 for more details

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))

Deviations:

yes

Remarks:

Food was provided on day 0 instead of day 1 due to good experience with this procedure. The room temperature increased to 23 °C for about 7.5 hours. At test end, the soil moisture deviated by more than 10 % from the initial value in all treatments.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 1452486
- CAS number: 68909-82-0
- Active ingredient: Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt
- Content (certified): 100% (UVCB)
- Appearance: Tan, powder (solid)
- Water solubility: Soluble
- Relative Density: No data available
- pH (5% solids): 9.5
- Expiration date of the lot/batch: 2021-06-07

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Keep dry. Store in original container at room temperature. Keep container tightly closed.
- Solubility in water: Soluble

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

On day -1, for homogenization, the respective test item amount for each concentration was weighed, dispersed in demineralised water and added to the artificial soil. Additional demineralised water was added to adjust the humidity of the artificial soil to a moisture of 54% of the WHCmax.
Subsequently, the test medium was thoroughly mixed to ensure a homogenous distribution, was covered to prevent any evaporation and stored in the dark under test conditions. On day 0, after further mixing, a soil amount corresponding to about 600 g soil dry weight was filled into test vessels.

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Eisenia fetida (Annelida, Lumbricidae)
Reason for the selection:
Eisenia fetida is suitable for this kind of study and is one of the recommended species according to the guideline.
Source:
Breeding stock culture maintained at the test facility
Breeding:
Organisms of the species Eisenia fetida are bred at the test facility in covered plastic vessels containing potting compost.
Synchronisation of the earthworm population:
Earthworms of homogeneous age and body weight were used in the definitive test. Synchronisation of the population was achieved by placing adult earthworms into breeding boxes and removing the adults after 4 weeks. Offspring from the remaining cocoons reached the adult age after 2 months at the earliest.
Feeding:
During breeding, the earthworms are fed with a litter of dried stinging nettle leaves and porridge oats. A sufficient amount of the food was provided depending on the feeding rate and the density of the earthworm population in the vessels.

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

8 wk

Test temperature:

Nominal: 20 +/- 2 °C
Actual: 18 - 23 °C
The room temperature increased to 23 °C for about 7.5 hours. This deviation is considered to have no impact on quality and integrity of the study.

pH:

Application rate
[mgNaphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis.,sodium salt/kg SDW] pH-value
day 0 day 56
Control 5.99 5.97
15.625 5.91 5.91
31.25 5.88 5.77
62.5 5.86 5.79
125 5.80 5.85
250 5.76 5.82
500 5.71 5.83

Moisture:

Application rate
[mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis.,sodium salt/kg SDW] Moisture [%] of dry weight WHCmax of artificial soil
[g/100 g DW]
day 0 day 56
Control 19.2 23.2* 35.5
15.625 19.0 24.5*
31.25 19.0 25.1*
62.5 18.9 24.5*
125 19.0 24.0*
250 18.8 24.5*
500 18.7 23.9*
* = The soil moisture deviated by more than 10 % from the initial value. This deviation is considered to have no impact on quality and integrity of the study.

Details on test conditions:

Experimental Procedure
Test item Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis.,sodium salt

Application rates
15.625 – 31.25 – 62.5 – 125 – 250 – 500 mg/kg SDW

Control
Artificial soil moistened with demineralised water without test or reference item was used as control medium

Test duration
8 weeks

Number of replicates
8 replicates per control and 4 replicates per test item concentration

Number and age ofthe earthworms
10 adult earthworms (with clitellum) aged 2-12 months were used per replicate. The difference of age did not deviate by more than 1 month (see “Synchronisation”).

Body weight (actual)
0.41 to 0.56 g

Adaptation
Earthworms were adapted to the artificial soil to be used for the test two days prior to test start.

Homogeneity of groups
The worms were washed with demineralised water and dried gently on a paper towel. The homogeneity of the population was checked by weighing the earthworms. After confirmation of homogeneity, groups of 10 earthworms (chosen by a randomised procedure) were weighed and introduced into each test vessel.

Test medium
Artificial soil consisted of the following components:
- 5 % peat, air-dried and finely ground
- 20 % kaolin, kaolinite content > 30 %
- 74 % air-dried quartz sand (sand with > 50 % particle size of 0.05 - 0.2 mm)
- 0.20 % calcium carbonate (CaCO3) to achieve a pH of 6.0  0.5

The WHCmax, the moisture content and the pH-value of the artificial soil were determined and adjusted. Two days before test start the artificial soil (22000 g) was pre-moistened with 1892 g demineralised water to achieve a moisture content corresponding to 27 % of the WHCmax of the artificial soil. The moistened soil was kept covered to prevent evaporative water losses prior to the start of exposure.

Test container
Test units containing about 600 g soil dry weight were round plastic boxes with an inner diameter of 15.0 cm corresponding to a bottom surface area of 177 cm2 and a height of 14 cm. Transparent and perforated lids enabled sufficient gas exchange, light input and prevented the test substrate from drying.

Application
On day -1, for homogenization, the respective test item amount for each concentration was weighed, dispersed in demineralised water and added to the artificial soil. Additional demineralised water was added to adjust the humidity of the artificial soil to a moisture of 54% of the WHCmax. Subsequently, the test medium was thoroughly mixed to ensure a homogenous distribution, was covered to prevent any evaporation and stored in the dark under test conditions. On day 0, after further mixing, a soil amount corresponding to about 600 g soil dry weight was filled into test vessels.

Temperature
20 +/- 2 °C

Photoperiod
16 h

Light intensity
400 - 800 lx

Feeding
The earthworms were fed with air-dried cattle manure weekly during the first 4 weeks as presented in Table 3. At test start and at day 28 the food was mixed carefully into the soil, on the other days the food was placed onto the soil surface.
The cattle manure was delivered by LEHR- UND FORSCHUNGSGUT RUTHE, Schäferberg 1, 31157 Sarstedt, Germany.

Moistening
Throughout the test the replicates were weighed weekly. Evaporated water was replenished by adding demineralised water to maintain the soil moisture which should not deviate by more than 10% of the initial value at the end of the test. Additional moistening was performed in case the soil appeared too dry.

Feeding and Additional Moistening During the Test
Day Amount of food [g]
Cattle manure Amount of demineralised water
[mL]
0 5 –
7, 14, 21, 28 5 10
35, 42, 49 – –

Nominal and measured concentrations:

15.625 – 31.25 – 62.5 – 125 – 250 – 500 mg/kg SDW

Reference substance (positive control):

yes

Remarks:

Carbendazim

Duration:

8 wk

Dose descriptor:

EC50

Effect conc.:

398 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

8 wk

Dose descriptor:

EC10

Effect conc.:

260 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

8 wk

Dose descriptor:

other: EC20

Effect conc.:

301 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

8 wk

Dose descriptor:

NOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

8 wk

Dose descriptor:

LOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

- Mortality at end of exposure period:
After 28 days of exposure in soil, neither evident earthworm mortalities (< 10%) nor pathological symptoms or changes in the behaviour of adult earthworms were observed in the control or all test item concentrations.
- Total mass of adults at beginning of test:
The earthworms had an individual body weight between 0.41 and 0.56 g at experimental starting.
- Changes in body weight of live adults (% of initial weight) at end of exposure period:
There were no statistically significant differences in earthworm body weight gains in the test item concentrations 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg soil dry weight compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg the earthworm body weight gain was statistically significantly reduced.
- No. of offspring produced:
The reproduction rate (average number of juveniles produced) was 127 in the control and ranged from 28 to 139 in the test item treatment rates. There were no statistically significant differences in earthworm reproduction in the treatment rates 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis.,sodium salt/kg SDW compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis.,sodium salt/kg the earthworm reproduction was statistically significantly reduced.

Results with reference substance (positive control):

Reference Item - Reproduction Rate (Number of Juveniles after 8 Weeks)
Application rate
[mg a.s./kg SDW] Rep. Number of juveniles Mean ± SD CV % of control Significance*
Control 1 35 36 ± 9.33 25.9 _ _
2 37
3 19
4 39
5 32
6 34
7 37
8 53
0.5 1 46 46 ± 2.45 5.33 128 No
2 43
3 46
4 49
1 1 28 44 ± 14.1 32.1 122 No
2 61
3 38
4 48
2 1 14 9 ± 5.32 59.1 25.0 Yes
2 3
3 12
4 5
4 1 0 0 ± 0.0 - 0.0 Yes
2 0
3 0
4 0

Rep. = Replicate a.s. = active substance SD = Standard deviation CV = Coefficient of variation
Sig. = Statistical Significance (ANOVA. α = 0.05) compared to control (Williams Multiple Sequential t-test Procedure)

According to OECD 222 significant effects of Carbendazim should be observed between 1 and 5 mg a.s./kg soil DW.

Reported statistics and error estimates:

Adult Mortality
The arithmetic mean and NOEC were determined. Since no mortality occurred the LCx – values were not determined.

Biomass, Reproduction
Significant differences in biomass and reproduction were determined in comparison with the control group by Williams Multiple Sequential t-test Procedure.
When running the test, a Shapiro-Wilk’s Test on Normal Distribution, a Levene’s Test on Variance Homogeneity (with Residuals) and a Trend analysis by Contrasts (Monotonicity of Concentration/Response) were done first. The alpha-value (acceptable probability of incorrectly concluding that there is a difference) was alpha = 0.05. For the endpoint of reproduction, the arithmetic mean and the variance (coefficient of variation) per treatment and control were calculated. The EC10,20,50 - values for reproduction were calculated.

Software
The data presented in the tables of the report were computer-generated and have been rounded for presentation. Thus, manual re-calculation of the data based on the results presented in this report may result in minor deviations from these figures.
Calculations were carried out using software
- Excel, MICROSOFT
- ToxRat Professional, TOXRAT SOLUTIONS GMBH

Adult Mortality

No evident earthworm mortality was observed in the controls and all test item concentrations.

 Mortality of Adult Earthworms in [%] after 28 Days of Exposure

Application rate		Replicate
[mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW]		1		2		3		4		5		6		7		8		Mean
Control		0		0		0		0		0		10		0		0		1.25
15.125		0		0		0		10		–					2.5
31.25		0		0		0		10						2.5
62.5		0		0		0		10						2.5
125		0		0		0		0						0
250		0		0		10		0						2.5
500		10		0		0		0						2.5

 

 

 Pathological Symptoms and Changes in Behaviour

No significant pathological symptoms or changes in the behaviour of adult earthworms were observed in the controls and all test item concentrations.

 

Earthworm Behaviour and Pathological Symptoms after 28 Days of Exposure

Application rate

Replicate

[mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis.,sodium salt/kg SDW]

Observation parameter

1

2

3

4

5

6

7

8

Control

A H

10/10 -

10/10 -

10/10 -

10/10 -

10/10 -

9/10 1/10

10/10 -

10/10 -

15.625

A H

10/10 -

10/10 -

10/10 -

9/10 1/10

-

31.25

A H

10/10 -

10/10 -

10/10 -

9/10 1/10

62.5

A H

10/10 -

10/10 -

10/10 -

9/10 1/10

125

A

10/10

10/10

10/10

10/10

250

A H

10/10 -

10/10 -

9/10 1/10

10/10 -

500

A H

9/10 1/10

10/10 -

10/10 -

10/10 -

A = no obvious pathological symptoms      H = earthworm dead

Live Weight

At test start, the earthworms had individual weights of 0.41 - 0.56 g. There were no statistically significant differences in earthworm body weight gains in the treatment rates 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW the earthworm body weight gain was statistically significantly reduced.
Body Weight Changes of the Adult Earthworms        

Application rate [mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt / kg SDW]	Replicate	Mean body weights per replicate		Mean body weight change of earthworms
		Test start	28 days	Per replicate		Mean ± SD		Sig.
		[g]	[g]	[g]	[%]	[g]	[%]
Control	1	0.46	0.59	0.13	28.3	0.11± 0.05	22.9 ± 10.3	–
	2	0.47	0.53	0.06	12.8
	3	0.46	0.60	0.14	30.4
	4	0.47	0.59	0.12	25.5
	5	0.46	0.50	0.04	8.7
	6	0.47	0.65	0.18	38.3
	7	0.46	0.58	0.12	26.1
	8	0.47	0.53	0.06	12.8
15.625	1	0.46	0.55	0.09	19.6	0.12 ± 0.03	25.2 ± 6.99	No
	2	0.47	0.60	0.13	27.7
	3	0.46	0.55	0.09	19.6
	4	0.47	0.63	0.16	34.0
31.25	1	0.46	0.49	0.03	6.5	0.07 ± 0.04	14.5 ± 8.27	No
	2	0.47	0.58	0.11	23.4
	3	0.46	0.55	0.09	19.6
	4	0.47	0.51	0.04	8.5
62.5	1	0.46	0.55	0.09	19.6	0.07 ± 0.06	15.6 ± 11.9	No
	2	0.47	0.52	0.05	10.6
	3	0.46	0.47	0.01	2.2
	4	0.47	0.61	0.14	29.8
125	1	0.46	0.56	0.10	21.7	0.11 ± 0.02	24.2 ± 3.32	No
	2	0.47	0.59	0.12	25.5
	3	0.46	0.59	0.13	28.3
	4	0.47	0.57	0.10	21.3
250	1	0.46	0.57	0.11	23.9	0.07 ± 0.04	15.1 ± 9.23	No
	2	0.47	0.48	0.01	2.1
	3	0.46	0.54	0.08	17.4
	4	0.47	0.55	0.08	17.0
500	1	0.46	0.52	0.06	13.0	0.07 ± 0.01	15.1 ± 2.49	Yes
	2	0.47	0.53	0.06	12.8
	3	0.46	0.54	0.08	17.4
	4	0.47	0.55	0.08	17.0

SD = Standard deviation                                                                       

Sig. = Statistical Significance (Williams Multiple Sequential t-test Procedure,α= 0.05) compared to control

Reproduction Rate

 

The reproduction rate (average number of juveniles produced) was 127 in the control and ranged from 28 to 139 in the test item treatment rates. There were no statistically significant differences in earthworm reproduction in the treatment rates 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg the earthworm reproduction was statistically significantly reduced. The EC₅₀-value for reproduction was determined to be 398 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW.

The coefficient of variation calculated for the reproduction of the control was 26.5 % and thus lower than 30 %. 

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study, Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt did not induce evident earthworm mortalities (< 10%) nor pathological symptoms or changes in the behaviour of adult earthworms in all test item concentrations. There were no statistically significant differences in earthworm body weight gains in the test item concentrations 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg the earthworm body weight gain was statistically significantly reduced. After a further four weeks, the reproduction rate (average number of juveniles produced) was 127 in the control and ranged from 28 to 139 in the test item treatment rates. There were no statistically significant differences in earthworm reproduction in the treatment rates 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg the earthworm reproduction was statistically significantly reduced. Overall, the NOEC of the test item concerning mortality, biomass and reproduction was determined to be 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW. The EC50-value for reproduction was determined to be 398 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.

Executive summary:

Effects of Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt on mortality, biomass and the reproductive potential of the earthworm species Eisenia fetida (Annelida, Lumbricidae) were determined according to OECD 222 (2016) from12 January to 10 March, 2017 with the definitive exposure phase from 13 January to 9/10 March, 2017 at the test facility in 31157 Sarstedt, Germany.

 The study was conducted under static conditions over 8 weeks with the test item concentrations 15.625 – 31.25 – 62.5 – 125 – 250 – 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW

Each application ratewas mixed into artificial soil containing 5 % peat. A control using untreated artificial soil was tested under the same conditions as the test item treatments. 80 test organisms were inserted into 8 control replicates and 40 test organisms were divided into 4 replicates for each treatment (10 earthworms per replicate). They had an individual body weight between 0.41 and 0.56 g at experimental starting.

 After 28 days of exposure in soil, neither evident earthworm mortalities (< 10%) nor pathological symptoms or changes in the behaviour of adult earthworms were observed in the control or all test item concentrations. There were no statistically significant differences in earthworm body weight gains in the test item concentrations 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg soil dry weight compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kgthe earthworm body weight gain was statistically significantly reduced. After a further four weeks, the reproduction rate (average number of juveniles produced) was 127 in the control and ranged from 28 to 139 in the test item treatment rates. There were no statistically significant differences in earthworm reproduction in the treatment rates 15.625 to 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW compared to the control. However, at the test item concentration 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg the earthworm reproduction was statistically significantly reduced. Overall, the NOEC of the test item concerning mortality, biomass and reproduction was determined to be 250 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW. The EC50-value for reproduction was determined to be 398 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.

 

All validity criteria recommended by the test guidelines were fulfilled.

Summary of All Observed Effects in theApplication rates

Effects	Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt
Application rates [mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW]	15.125	31.25	62.5	125	250	500
Mortality of adult earthworms	No	No	No	No	No	No
Body Weight Gain	No	No	No	No	No	Yes
Reduced reproduction rate (Number of juveniles)	No	No	No	No	No	Yes
Summary of all effects	No	No	No	No	No	Yes

NOEC, LOEC and EC-values

Endpoint	[mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW]
LOECmortality	>500
NOECmortality	500
LOECreproduction, biomass	500
NOECreproduction, biomass	250
EC-valuesreproduction (95 % Confidence Interval)	EC10: 260 (not determinable) EC20: 301 (not determinable) EC50: 398 (not determinable)

 

Description of key information

No terrestrial ecotoxicity tests have been performed with Naphthalenesulfonic acid, methyl, butyl, sodium salt (CAS no.:68909-83-1). The results from the structural analogue Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt is therefore read across.

Effects of Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt on mortality, biomass and the reproductive potential of the earthworm species Eisenia fetida (Annelida, Lumbricidae) were determined according to OECD 222 (2016).       

The study was conducted under static conditions over 8 weeks with the test item concentrations 15.625 – 31.25 – 62.5 – 125 – 250 – 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW.

Each application rate was mixed into artificial soil containing 5 % peat. A control using untreated artificial soil was tested under the same conditions as the test item treatments.

After 28 days of exposure in soil, neither evident earthworm mortalities (< 10%) nor pathological symptoms or changes in the behaviour of adult earthworms were observed in the control or all test item concentrations. There were no statistically significant differences in earthworm body weight gains in the test item concentrations 15.625 to 250 mg test item/kg soil dry weight compared to the control. However, at the test item concentration of 500 mg/kg the earthworm body weight gain was statistically significantly reduced. After a further four weeks, the reproduction rate (average number of juveniles produced) was 127 in the control and ranged from 28 to 139 in the test item treatment rates. There were no statistically significant differences in earthworm reproduction in the treatment rates 15.625 to 250 mg test item /kg SDW compared to the control. However, at the test item concentration 500 mg test item/kg the earthworm reproduction was statistically significantly reduced.

Overall, the EC₁₀of the test item concerning reproduction was determined to be 260 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt /kg SDW. The EC50-value for reproduction was determined to be 398 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.  

All validity criteria recommended by the test guidelines were fulfilled.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:

250 mg/kg soil dw

Additional information

The EC50-value for reproduction was determined to be 398 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.

The NOEC-value for mortality was 500 mg Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt/kg SDW.

No mortality was observed at 1000 mg/kg SDW in the 14 day (non-GLP) range finder.