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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes
Specific details on test material used for the study:
Test material:
IR5878
Batch number: FCF/T/168-00 (ex 20525/03/9)
Purity: 98.54 ± 0.51 %

Reference item: Aniline p.A., (purity = 99.5% freshly distilled)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, adapted
Duration of test (contact time):
ca. 28 d
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
IR5878 was investigated for its ready biodegradability in a closed bottle test over a period of 28 days. The biodegradability was followed by the oxygen uptake of the micro organisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as IR5878, and functioned as a procedure control.
Reference substance:
aniline
Key result
Parameter:
% degradation (O2 consumption)
Value:
ca. 0
Sampling time:
28 d
Details on results:
Under the test conditions the percentage biodegradation of IR5878 was determined to be 0% after 28 days of incubation, based on ThODNH4 and ThODNO3.
IR5878 can therefore be considered to be not ready biodegradable.
The reference item Aniline was sufficiently degraded to a mean of 72% after 14 days, and to a mean of 91% after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Based on the results of a biodegradability study, IR5878 is not a readily biodegradable substance.
Executive summary:

Under the test conditions the percentage biodegradation of IR5878 was determined to be 0% after 28 days of incubation, based on ThODNH4and ThODNO3.

IR5878 can therefore be considered to be not ready biodegradable.

The reference item Aniline was sufficiently degraded to a mean of 72% after 14 days, and to a mean of 91% after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.

Description of key information

Under the test conditions the percentage biodegradation of IR5878 was determined to be 0% after 28 days of incubation, based on ThODNH4and ThODNO3.

IR5878 can therefore be considered to be not ready biodegradable.

The reference item Aniline was sufficiently degraded to a mean of 72% after 14 days, and to a mean of 91% after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.

 

Conclusions

Based on the results of a biodegradability study, IR5878 is not a readily biodegradable substance.

 

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information