Absolute of Boswellia Carterii (Burseraceae) obtained from exudate by organic solvents treatment and subsequent ethanol extraction

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 November 2017 - 11 January 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method. Test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 13 and 14 October 2014/ signed on 8 april 2015

Type of method:

pycnometer method

Key result

Type:

relative density

Density:

1.051 other: no dimension

Temp.:

20 °C

All measured values are given in the following table:

First Determination
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn.+ H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	43.4350	94.3164	94.3217	53.2900	94.8540
Temperature in °C	20.0	19.8	20.2	20.0	19.6
Second Determination
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn.+ H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	43.4345	94.3079	94.3363	52.8407	94.7655
Temperature in °C	19.6	20.0	19.8	20.0	20.2

The following values were calculated.

First Determination			Error rel.	Error abs.
Temperature	19.8	°C		0.1
Density of water	0.998203	g/cm^3	2.00%	0.0002
Volume of Pycnometer	50.9730	cm^3
Density of Replacement Liquid	0.9983	g/cm^3
Density of Test Item	1.0553	g/cm^3	0.0213%	0.0002
Second Determination			Error rel.	Error abs.
Temperature	20.0	°C		0.1
Density of water	0.998203	g/cm^3	2.00%	0.0002
Volume of Pycnometer	50.9650	cm^3
Density of Replacement Liquid	0.9988	g/cm^3
Density of Test Item	1.0465	g/cm^3	0.0221%	0.0002

Results:

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	50.97	cm^3	0.0057	0.01 %
Density of Test Item	1.0509	g/cm^3	0.0062	0.59 %
Density of Test Item	1050.9	kg/m^3	6.2	0.59 %
Relative Density of Test Item	1.0509	-	0.0062	0.59 %

Conclusions:

The density of the test item was calculated as 1.0509 ± 0.0002 g/cm3 at 20.0 ± 0.4 °C with the maximum error of a single determination.
Standard deviation was 0.0062 g/cm3, showing acceptable correlation of the two determinations.
Therefore, the study is considered valid.

Executive summary:

The study was performed in order to determine the density of test item according to OECD 109, resp. EU A.3 guideline, using a pycnometer.

The density of the test item was calculated as 1.0509 ± 0.0002 g/cm3 at 20.0 ± 0.4 °C

Description of key information

The density of test item was determined to be 1.0509 ± 0.0002 g/cm³ at 20±0.4 °C.

This value is the mean of two independent determinations.

Key value for chemical safety assessment

Relative density at 20C:

1.051

Additional information

A fully reliable experimental study, conducted according to OECD 109 /EU A3 guideline, using pycnometer, is available. It is considered as a key study, and the result is retained as key data.