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EC number: 249-636-2 | CAS number: 29450-45-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25.11.2008 - 27.11.2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-ethylhexyl (4-chloro-2-methylphenoxy)acetate
- EC Number:
- 249-636-2
- EC Name:
- 2-ethylhexyl (4-chloro-2-methylphenoxy)acetate
- Cas Number:
- 29450-45-1
- Molecular formula:
- C17H25ClO3
- IUPAC Name:
- 2-ethylhexyl (4-chloro-2-methylphenoxy)acetate
- Reference substance name:
- (4-chloro-2-methylphenoxy)acetic acid
- EC Number:
- 202-360-6
- EC Name:
- (4-chloro-2-methylphenoxy)acetic acid
- Cas Number:
- 94-74-6
- Molecular formula:
- C9H9ClO3
- IUPAC Name:
- (4-chloro-2-methylphenoxy)acetic acid
- Test material form:
- liquid
Constituent 1
impurity 1
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.437 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 0.437 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 0.437 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.437 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 0.437 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 0.437 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
Any other information on results incl. tables
RESULTS AND DISCUSSION
THE PRELIMINARY TEST
According to the information from the Sponsor the test substance is insoluble in water (The test item description, Annex No 1 to contract No 23/PP/08). According to literature data the estimated limit of MCPA-2EH solubility is <0.17 mg/L and the ester is rapidly hydrolyzed. The test substance of low solubility should be tested up to the limit of solubility or at the concentration above the limit of solubility to ensure that the maximum soluble concentration has been obtained.
The preliminary test was performed in five concentrations: 0.10; 0.18; 0.32; 0.56 and 1.00 mg/L. In order to obtain each test concentration the same amount 0.1 mL of test item in acetone (solutions of certain concentrations). The time between test item addition into water and extraction into organic solvent (sample preparation for chemical analysis) was no longer than 30 min.
The analytical results obtained confirm the correct test item application. The detected contents of MCPA-2EH in test concentrations at the test beginning range 80.0 – 100.3 % of nominal.
Table 4. MCPA-2EH – Concentration of (4-chloro-2-methylphenoxy)acetic acid 2-ethylhexyl ester in the preliminary test
Nominal test item |
Mean concentration (n = 3) of MCPA-2EH |
|
t0 |
% of nominal |
|
Control |
-- |
|
0.10 |
0.082 |
82.43 |
0.18 |
0.156 |
86.76 |
0.32 |
0.321 |
100.25 |
0.56 |
0.448 |
80.02 |
1.00 |
0.868 |
86.80 |
LOD
= 0.05 mg/L
LOQ = 0.10 mg/L
Based on biological results obtained it was demonstrated that test item did not cause any immobilization at the tested range of concentrations (Table 5).
Table 5. MCPA-2EH – Daphnia magna acute immobilization test, the preliminary test
Concentration |
Number of |
Number of dead or immobilized |
Total percentage of immobilized |
||||||||
24 h |
48 h |
||||||||||
Replicates |
|||||||||||
A |
B |
C |
D |
A |
B |
C |
D |
24 h |
48 h |
||
Control |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.10 |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.18 |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.32 |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.56 |
20 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
5 |
1.00 |
20 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
THE DEFINITIVE TEST
The definitive test was performed at one test substance concentration above the estimated limit of solubility in water. Acetone was used at 0.1 mL/L concentration as auxiliary substance to prepare the test concentration. Therefore, in the definitive test both standard control and control with acetone were conducted.
VALIDITY CRITERIA
The following validity criteria were met:
· Mortality and immobilization ofDaphnia magnain control was 0 % (allowed 10% [1, 2]),
· Concentration of dissolved oxygen was higher than 3 mg/L (should be kept at ≥3 mg/L).
DEVIATIONS FROM GUIDELINE
No deviations from Guideline occurred in the definitive test performed.
All validity criteria of the definitive test mentioned in both OECD Guideline No 202 and test
method EU C.2. were met.
ENDPOINTS
The acute toxicity ofMCPA-2EHforDaphnia magnawas studied in limit test at one test concentration 1.0 mg/L, i.e. above the estimated limit of solubility, to ensure the maximum stable concentration in water.
The contents ofMCPA-2EHwere chemically analyzed in the definitive test. The test substance was detected in the test concentration and the control with acetone at the beginning and the end of exposure (t0 andt48).Results of concentration and stability measurements performed are presented in Table 6.
Table 6.
MCPA-2EH – Concentration and stability of
(4-chloro-2-methylphenoxy)acetic acid
2-ethylhexyl ester in the definitive test
Nominal test item |
Mean concentration (n = 3) of MCPA-2EH measured |
Geometric mean of |
|||
t0 |
% of nominal concentration |
t48 |
% of initial concentration |
||
Control |
<LOD |
-- |
<LOD |
-- |
-- |
1.0 |
0.885 |
88.50 |
0.216 |
24.41 |
0.437 |
In the sample collected at the start of the test the content of MCPA-2EH was 88.5 % of nominal. This confirms that the test item was correctly applied.
The test substance is not stable in the test concentration during 48 h of exposure. The content of test substance detected at the test end was 24.4 % of initial concentration.
The toxicity of MCPA-2EH forDaphnia magnawas assessed based on immobilization observations during the test.
In the test concentration 1.0 mg/L none of test organisms was immobile. In the control and the control with acetone no immobilization was observed.
The ECxvalues were estimated based on nominal test item concentration, because the analytical results confirmed its correct application. The ECxvalues are presented in Table 7. Median concentration causing immobilization of 50 % population (EC50) after 48 hours of exposure is above 1.0 mg/L. The EC0value after 48 h is equal or above 1.0 mg/L andEC100is above 1.0 mg/L.
Based on the chemical analyses performed the content of MCPA-2EH is not stable in test conditions. Therefore, the geometric mean of measured test substance concentrations was calculated. The ECxvalues were also determined based on the geometric mean Median concentration causing immobilization of 50 % population (EC50) after 48 hours of exposure is above 0.437 mg/L. The EC0value after 48 h is equal or above 0.437 mg/L and EC100is above 0.437 mg/L.
Applicant's summary and conclusion
- Executive summary:
The study was performed according to the OECD Guidelines for Testing of Chemicals No 202 (2004) and EU method C.2. as well as in compliance with Principles of GLP.
The aim of the study was evaluation of acute toxicity of test item for Daphnia magna and determination of EC50, EC0and EC100values after 24 h and 48 h.
The test item was used in one concentration 1.0 mg/L (limit test). The content of test substance was determined at the beginning and at the end of exposure. The results of chemical analyses confirmed the correct application of test item. The detected substance is not stable in experiment conditions.
Taking into account the obtained results after 24 and 48 hours of exposure the EC50 value is above 1.0 mg/L, EC0 is equal or above 1.0 mg/L and EC100 is above 1.0 mg/L based on test item nominal concentrations.
Based on geometric mean of determined test substance concentrations the EC50 value is above 0.437 mg/L, EC0 is equal or above 0.437 mg/L and EC100 is above 0.437 mg/L.
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