3,7-dimethyloctane-1,7-diol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16.02.1998 to 20.02.1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

GLP compliance:

yes

Remarks:

Department of Toxicology of BASF Aktiengesellschaft, 67056 Ludwigshafen/Rhein (Germany)

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dimethyloctane-1,7-diol

EC Number:

203-517-1

EC Name:

3,7-dimethyloctane-1,7-diol

Cas Number:

107-74-4

Molecular formula:

C10H22O2

IUPAC Name:

3,7-dimethyloctane-1,7-diol

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: BASF AG, Batch No.: 2/10/97
- Expiration date of the lot/batch: not reported
- Purity test date: not reported

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, exclusion of light
- Stability under test conditions: yes
- Solubility and stability of the test substance in the solvent/vehicle: water solubility: 30.1 g/L at 25°C, stability under test conditions was proved by reanalysis

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no
- Preliminary purification step (if any): no

FORM AS APPLIED IN THE TEST (if different from that of starting material): aqueous solution

Sampling and analysis

Analytical monitoring:

yes

Remarks:

GC/FID

Details on sampling:

- Concentrations: 0, 21.5, 46.4, 100, 215, 464, 1000, 2150 g/L nominal
- Sampling method: extraction of the aqueous phase using dichloromethane

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish (Brachydanio rerio Ham. and Buch.)
- Source: Bio International B.V., NJ Horn; Netherlands; For the added concentrations: Bio International B.V., Roermond; Netherlands
- Age at study initiation (mean and range, SD): ca. 6 months
- Length at study initiation (length definition, mean, range and SD): for the added concentrations: 3.07 (2.6-3.4) cm
- Weight at study initiation (mean and range, SD): for the added concentrations: 0.173 (0.10-0.30) g
- Method of breeding: The fish were kept in a flow-through tank in tap water, not chlorinated, passed through a charcoal filter and aerated with oil-free air

ACCLIMATION
- Acclimation period: 1 month
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: TETRA MIN standard feed for aquarium fish, TETRA-Werke, Melle, Germany, ad libitum; and on workdays generally additionally live brine shrimp (artemia)
- Feeding frequency during acclimation: TETRA MIN standard feed for aquarium fish, TETRA-Werke, Melle, Germany, ad libitum; and on workdays generally additionally live brine shrimp (artemia)
- Health during acclimation (any mortality observed): mortality during the last 14 d: 1.5%; for the added concentrations: 0%

FEEDING DURING TEST
- Food type: TETRA MIN standard feed for aquarium fish, TETRA-Werke, Melle, Germany, ad libitum; and on workdays generally additionally live brine shrimp (artemia)
- Amount: ad libitum; and on workdays generally additionally live brine shrimp (artemia)
- Frequency: ad libitum; and on workdays generally additionally live brine shrimp (artemia)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

250 mg/L CaCO3

Test temperature:

22°C

pH:

8.3-8.6 (in all test vessels)

Dissolved oxygen:

7.5-9.0 (in all test vessels)

Nominal and measured concentrations:

nominal:
0, 21.5, 46.4, 100, 215, 464, 1000, 2150 mg/L
measured
after 1 h: 0, 20.85, 43.35, 99.5, 228, 442, 964, 2182 mg/L (mean of two measurements)
m

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Type (delete if not applicable): open / closed not reported
- Material, size, headspace, fill volume: glass/15.84 L/-/10 L
- Aeration: yes
- Type of flow-through (e.g. peristaltic or proportional diluter): test conducted under static conditions
- Renewal rate of test solution (frequency/flow rate): test conducted under static conditions
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.2 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter aerated
- Intervals of water quality measurement: intervals not reported (water quality was checked regularly)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, behaviour/symptoms

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: performed
- Test concentrations: not reported
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 464 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

198 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 415.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

986.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: at concentrations >=464 mg/L nominal and exposure times >24 h, apathy, convulsion, and tumbling were observed
- Mortality of control: 0
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Reported statistics and error estimates:

Probit analysis was performed for calculation of toxicity values (NOEC, LC).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity test with fish, 96 h LC50 values based on nominal and measured concentrations were determined to be ca. 464 and 415.5 mg/L, respectively.

Executive summary:

The acute toxicity of hydroxycitronellol to fish was investigated in a study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test; 1992) and EU Method C.1 (Acute Toxicity for Fish; 1992). 10 Fish were exposed to 8 nominal concentrations ranging from 0 to 2150 mg/L for 96 h under static conditions. Mortality and behaviour/symptoms were recorded. The test substance concentrations were measured via GC/FID. The analytically determined concentrations were initially in the range of 93 -108% and after about 96 h between 73 and 100% of the nominal concentrations. The 96 h LC50 values based on nominal and measured concentrations were determined to be ca. 464 and ca. 415.5 mg/L, respectively.

The study is well documented and can be classified as reliable without restrictions satisfying the guideline requirements for an acute toxicity study with fish.

Results Synopsis

Test organism size/age: Brachydanio rerio (new name: Danio rerio; 6 months old, length: 3.07 cm; weight: 0.173 g)

Test Type: static

96 h LC50: ca. 464 mg/L (nominal)

96 h LC50: ca. 415.5 mg/L (measured)

Endpoint(s) Effected: mortality, behaviour/symptoms