2-butoxyethyl benzoate

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants, Growth Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-butoxyethyl benzoate

EC Number:

226-685-8

EC Name:

2-butoxyethyl benzoate

Cas Number:

5451-76-3

Molecular formula:

C13H18O3

IUPAC Name:

2-butoxyethyl benzoate

Test material form:

other: liquid

Details on test material:

2-Butoxyethyl benzoate, Lot # 201303443-19; Purity 99.2 %

Specific details on test material used for the study:

2-Butoxyethyl Benzoate (Lot Number 201303443-19)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

After preparation of dosing stocks, 10 mL samples were collected to verify test concentration and to demonstrate homogeneity of the test substance in the dosing solutions. A single sample was collected from the negative control, the solvent control, and the lower four test level dosing stocks. Triplicate samples were collected from the high level dosing stock. Following collection, samples were transferred to the analytical chemistry laboratory for analysis.

Test substrate

Vehicle:

yes

Remarks:

acetonitrile

Details on preparation and application of test substrate:

Test soils were prepared by mixing stock solutions of the test substance into 40 kg fresh weight of soil. A primary stock was prepared by adding 75.1020 g of the test substance to 1000 mL of acetonitrile and mixing by swirling, inversion and stirring. The primary stock concentration was based on a mean, measured soil moisture content of 8%, and a volume of 490 mL to be used for soil preparation. Secondary stocks were prepared by diluting 31.3, 62.5, 125 and 250 mL aliquots of the primary stock, each to a final volume of 500 mL with acetonitrile, and mixing by stirring, swirling, and inversion. The solvent control stock consisted of acetonitrile only, and the negative control stock consisted of well water purified by reverse osmosis. In order to prepare test soils, 490 mL of eachstock was added to 40 kg of bulk soil, mixed in a soil mixer for approximately 10 minutes, turned by hand, and then mixed for another approximately 10 minute period. The negative control was prepared first, followed by the solvent control, and then the test soils in order of increasing concentration to avoid cross-contamination of the test material.

Test organisms

1

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

Glycine max (soybean) Viking 2265
Fabaceae
Johnny’s Selected Seeds, Winslow, ME USA
88

2

Species:

Zea mays

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

Zea mays (corn) Nothstine Dent OG
Poaceae
Johnny’s Selected Seeds, Winslow, ME USA
98

Study design

Test type:

seedling emergence and seedling growth test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

21 d

Test conditions

Nominal and measured concentrations:

63, 125, 250, 500 and 1000 milligrams test substance per kilogram of soil dry weight (mg/kg). Measured concentrations were within 99 - 101% of nominal.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 2

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Effect concentrations 3

Key result

Species:

Zea mays

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 4

Key result

Species:

Zea mays

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Details on results:

Test soil concentrations of up to 1000 mg/kg dry soil resulted in no adverse effects on the seedling emergence or survival of either Z. mays or G. max. There were adverse effects on height and dry weight of both Z. mays and G. max at the highest treatment levels. The NOEC and LOEC for Z. mays (corn) were 500 and 1000 mg/kg dry soil respectively for both dry weight and height. The NOEC and LOEC for G. max (soybean) were 250 and 500 mg/kg dry soil respectively for both dry weight and height.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

Test soil concentrations of up to 1000 mg/kg dry soil resulted in no adverse effects on the seedling emergence or survival of either Z. mays or G. max. There were adverse effects on height and dry weight of both Z. mays and G. max at the highest treatment levels. The NOEC and LOEC for Z. mays (corn) were 500 and 1000 mg/kg dry soil respectively for both dry weight and height. The NOEC and LOEC for G. max (soybean) were 250 and 500 mg/kg dry soil respectively for both dry weight and height.