Registration Dossier
Registration Dossier
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EC number: 277-155-8 | CAS number: 72968-81-1
Endpoint summary
Administrative data
Description of key information
EC50 (48h) invertebrate Daphnia: > 100 mg/L
EC50 (7d) aquatic plants Lemna minor: > 0.69 mg/L (frond number, dry weight). No acute toxicity (i.e. ErC50).
Additional information
Invertebrate acute toxicity
The acute toxicity of the Similar Substance 01 to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202. The nominal test concentrations applied were 1, 10 and 100 mg/L. TheEC50 (24h) was found > 100 mg/L (nominal).
The acute toxicity on Daphnia (Immobilisation) following OECD 202 was tested also on a Similar substance 02. The test concentrations were 0.1, 1, 10, 100 and 1000 mg/L of dye for a total exposure of 48h. The tested sample under the experimental conditions showed an EC50,48h = 17.8 mg/L.
Aquatic Plants toxicity
The effect of test item was assessed on Lemna gibba over an exposure period of 7 days, following the testing procedures outlined into the OECD guideline 221 on a Similar substance 01.
The 7-d ErC50, based on both frond number and dry weight, was determined to be higher than 0.69 mg/L. No acute toxicity (i.e. ErC50) was recorded up to the higher concentration reached in the test. The decrease of the measured concentrations during the experiment was due to the test substance adsorption to the lemna roots.
The Similar substance 02 was tested for acute toxicity to algae following OECD 201. Under the experimental conditions the IC50,72h is equal to 12.8 mg/L.
Considering that the test substance could influence the photosynthesis capacity of algae cells in the test solution, it was considered a test using Lemna gibba a test organism.
Based on the read-across principle, the results can be considered for the assessment of the registered substance.
Justification for read-across is detailed in the report attached to the IUCLID section 13.
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