2-[4-(cyclopropanecarbonyl)phenyl]-2-methyl-propanenitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-(4-cyclopropanecarbonylphenyl)-2-methylpropanenitrile

EC Number:

812-121-3

Cas Number:

169280-06-2

Molecular formula:

C14 H15 N O

IUPAC Name:

2-(4-cyclopropanecarbonylphenyl)-2-methylpropanenitrile

Sampling and analysis

Analytical monitoring:

yes

Remarks:

HPLC-UV

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna, female, clone 5; 2 to 21 hours old.
The daphnids introduced in the test were taken from ibacon's in-house laboratory culture.

Study design

Test type:

static

Water media type:

other: reconstituted water (Elendt M4)

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Visual observation after 24h and 48h of the mobility of animals after agitation of the test beaker.

Test conditions

Hardness:

2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

19.0-19.8°C (water temperature)

pH:

7.8-8.3

Dissolved oxygen:

8.7-9.1 mg/L

Nominal and measured concentrations:

A supersaturated stock solution of nominal 100 mg test item/L was prepared by suspending 100.0 mg test item in 1000 mL test water.
Due to the low limit of water solubility of the test item, a filtrate of nominal 100 mg/L and dilutions of 1:2, 1:4, 1:8 and 1:16 of this filtrate were tested. The filtrate and its dilutions are corresponding to following arithmetic mean measured concentrations of the test item: 32.2, 16.0, 7.76, 3.98, 1.92 mg test item/L

Details on test conditions:

Photoperiod: 16h light- 8h dark; light intensity: 370-790 lux.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 10.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 7.76 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 16 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

After 48 hours of exposure, no immobilisation of the test animals was observed in the control and the arithmetic mean measured concentrations of 1.92 and 7.76 mg test item/L. One immobile Daphnia was observed at the arithmetic mean measured concentration of 3.98 mg test item/L. Since no effect was observed at 7.76 mg test item/L anymore, the immobilisation at 3.98 mg test item/L was not considered to be treatment related. At the arithmetic mean measured concentrations of 16.0 and 32.2 mg test item/L, all animals were immobile after 48 hours of exposure.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

All reported results refer to mean measured values since the concentrations of the test item were within +/- 20% of the measured initial concentrations during the test.
Based on the experimental results, the 48-h NOEC was determined to be 7.76 mg test item/L. The 48-h LOEC was determined to be 16.0 mg test item/L and the 48-h EC50 value was calculated to be 10.3 mg test item/L.