Alkenes, C10-12, C11-rich

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study report which meets basic scientific principles: pre-GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1962

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

0.2 ml of test material was placed into the mouth of an anesthetized animal at which point the animal was stimulated to aspirate the material.

Test material

Test material information

Constituent 1

Reference substance name:

Decene

EC Number:

246-870-7

EC Name:

Decene

Cas Number:

25339-53-1

IUPAC Name:

dec-1-ene

Details on test material:

The olefins used in the study were hexene, octene, decene, dodecene, tetradecene, hexadecene, octadecene

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Administration / exposure

Route of administration:

other: aspiration

Vehicle:

unchanged (no vehicle)

Doses:

0.2 ml

No. of animals per sex per dose:

2 per dose

Details on study design:

Rats were anesthetized with ether, 0.2 ml of test material was placed into the mouth, and the animal was induced to aspirate the liquid by closing the nostrils with the thumb and forefinger during the inspiratory phase of the breathing cycle. After dosing, the animals were monitored for a minimum of 4 hours at intervals. Lungs were removed and examined as soon as possible after death. Twenty-four hours after dosing, survivors were euthanized and examined.

Statistics:

Not applicable

Results and discussion

Mortality:

Animals that aspirated hexene died within 2 minutes. All other compounds induced death within 1 hour of aspiration.

Clinical signs:

Not applicable

Body weight:

Not applicable

Gross pathology:

Some lungs were dark red.

Applicant's summary and conclusion

Conclusions:

The aspiration toxic potential of hexene, octene, decene, dodecene, tetradecene, hexadecene, and octadecene is 100%. This finding warrants the classification of higher olefins as aspiration hazards under EU GHS guidelines and warrants classification under the EU requirements for dangerous substances and preparations guidelines.

Executive summary:

Hexene, octene, decene, dodecene, tetradecene, hexadecene, and octadecene were administered via induced aspiration to 2 male rats at a dose of 0.2 ml to assess the aspiration hazard.  At the end of the study, except for hexene which induced death within 2 minutes of exposure, all other olefins tested induced death within 1 hour. This finding warrants the classification of higher olefins as aspiration hazards under EU GHS guidelines and warrants classification under the EU requirements for dangerous substances and preparations guidelines.