Sodium [4-[[dihydroxy[(2-hydroxy-3,5-dinitrophenyl)azo]phenyl]azo]benzenesulphonato(3-)]cuprate(1-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Oct 25, 2016 - Dec 19:2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD 202:2004

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: National Chiayi University Freshwater Biological Resource Center
- Age: less than 24 hours old were derived form the same stock and were not first progeny.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 °C

Details on test conditions:

- Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.57 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

In range-finding test, the 0% of control organisms were immobilised at the end of the test and the dissolved oxygen concentration of control and test groups during the test was 8.29-8.44 mg O2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. The results of the range-finding test showed that EC₅₀ was 0.24 mg/L.

In definitive test, the results showed below:

 

Table 1. The numbers immobilized forthe definitive teston day 1 and 2

Days	Group (mg/L)		Numbers of no immobilised	Numbers of activity immobilised	Numbers of discoloration	Numbers of trapping at surface of water
Day1	Control group	0.00	20	0	0	0
	Test group	4.00	8	12	0	0
		1.82	13	7	0	0
		0.83	17	3	0	0
		0.38	20	0	0	0
		0.17	20	0	0	0
		0.08	20	0	0	0
		0.04	20	0	0	0
Day2	Control group	0.00	20	0	0	0
	Test group	4.00	5	15	0	0
		1.82	12	8	0	0
		0.83	17	3	0	0
		0.38	18	2	0	0
		0.17	19	1	0	0
		0.08	18	2	0	0
		0.04	20	0	0	0

Table 2. The percentages immobilized for the definitive test

Group (mg/L)		Percentages immobilized (%)
		Day 0	Day 1	Day 2
Control group	0.00	0.00	0.00	0.00
Test group	4.00	0.00	60.00	75.00
	1.82	0.00	35.00	40.00
	0.83	0.00	15.00	15.00
	0.38	0.00	0.00	10.00
	0.17	0.00	0.00	5.00
	0.08	0.00	0.00	10.00
	0.04	0.00	0.00	0.00

Table 3. EC50 and 95% confidence interval for the definitive test

Parameter	Concentration
	(mg/L)
EC50	2.57
95% confidence interval-lower	2.27
95% confidence interval-upper	2.92

Table 4. Temperature, dissolved oxygen and pH value for the definitive test

Group (mg/L)		Day 0			Day 2
		Temperature (°C)	Dissolved oxygen (mg/L)	pH value	Temperature (°C)	Dissolved oxygen (mg/L)	pH value
Control group	0.00	20.0	8.75 ± 0.14	7.49 ± 0.06	20.0	8.85 ± 0.04	6.88 ± 0.10
Test group	4.00		8.66 ± 0.09	7.57 ± 0.05		8.93 ± 0.05	7.02 ± 0.06

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 202 test method, the 48-hr EC50 of Everlan SL65 was 2.57 mg/L.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100108002EN which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for Everlan SL65 show that all validity criteria were met. The EC50 was 0.24 mg/L in the range-finding test, so the definitive test was conducted. The concentrations were 0.04, 0.08, 0.17, 0.38, 0.83, 1.82 and 4.00 mg/L in the definitive test. Under the conditions of this test, the 48-hr EC50 of Everlan SL65 was 2.57 mg/L.

Description of key information

The 48-hr EC50 of Everlan SL65 was 2.57 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.57 mg/L

Additional information

In both range-finding and definitive test, not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration of control and test groups at the end of the test was ≥3 mg O₂/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Based on the results derived from the range-finding test, there should conduct definitive test. Therefore, the concentrations set between 0.04 and 4.00 mg/L in the definitive test. Under the conditions of this test, the 48-hr EC50 of Everlan SL65 was 2.57 mg/L.