3',4',5'-trifluorobiphenyl-2-amine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): 3',4',5'-Trifluoro-biphenyl-2-ylamine
- Physical state: Solid / dusky pink up to red
- Analytical purity: 99.67 area-%
- Lot/batch No.: 33569-66 eing.2
- Expiration date of the lot/batch: Unlimited
- Storage condition of test material: Storage at room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analysed. The samples were transported to the Analytical Laboratory on the day of sampling.

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Prior to use the test substance was homogenized with a pestle. For correct dosing of the test substance acetone was used as solvent. For each test concentration the appropriate volume of the acetonic solution of 50 mg test substance in 500 mL acetone was prepared. The acetonic solution was distributed on the bottom of the test vessel to maximize the surface and the acetone was completely evaporated. After this 400 mL test medium were added and the test vessels were shaken for about one day at the test temperature (at 50 rpm). The pH of the test solutions was not adjusted.
To ensure that the organisms were only exposed to dissolved test substance, the exposure was carried out in glass beakers into which were placed organism chambers consisting of a glass tube with a stainless steel mesh bottom. The glass tubes were submerged in beakers with test medium with the top of the tube sufficiently above the surface of the test medium. The bottom of the tube was suspended above the bottom of the test vessel preventing direct contact with the layer of undissolved test substance.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: Institut National de Recherche Chimique Appliquée, France, in 1978.
- Age at study initiation: < 24 hours (at least 3rd brood progeny)
- Feeding during test: None

CULTURE CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Culture media: Synthetic fresh water (Elendt M4) prepared according to OECD 202
- Temperature: 20 ± 1 °C
- Diet: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility
- Feeding: Daily
- Renewal of the culture water: Twice weekly

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 – 3.20 mmol/L

Test temperature:

20.3 – 21.1 °C (continuous monitoring)

pH:

7.5 – 8.5

Dissolved oxygen:

8.9 mg/L

Nominal and measured concentrations:

Nominal concentrations (mean measured concentrations in brackets): 0, 0.5, 1.0 (0.5103) , 2.2 (1.327), 5.0 (2.67), 10.0 (5.566) mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 600 mL) into which organism chambers were placed (cylinders of transparent glass, 6 cm diameter, 10 cm high, with a bottom of stainless-steel grid of 0.2 mm x 0.2 mm), filling volume of 400 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
- Biomass loading rate: 0.013 animals/mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Ca/mg ratio: about 4:1
- Conductivity: 550 - 650 μS/cm
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: About 20 – 850 lux at a wave length of 400 -750 nm

EFFECT PARAMETERS MEASURED: Immobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1.327 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.882 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

2.67 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Immobility of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None