1-methylpiperidin-4-ol

Endpoint summary

Description of key information

Short term toxicity to fish:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 106-52-5 on Zebra fish (Danio rerio)was found to be >100 mg/L.

Short term toxicity of aquatic invertebrate:
The predicted data by ECOSAR version 1.1, suggests the 48 hr lethal concentration (LC50) for the 4-Piperidinol, 1-methyl- (CAS no106-52-5) to be 3674.980mg/l for Daphnia magna.

Toxicity of aquatic algae and cynobacteria:
The median Effective concentration (EC50) value for 1-methylpiperidin-4-ol on Pseudokirchneriella subcapitata in a 72 hour study was estimated to be 110.386 mg/L on the basis of effects on growth rate.

Toxicity to microorganisms:
The Inhibitory growth concentration (IGC50) value for 1-methylpiperidin-4-ol for Tetrahymena pyriformis in a 48 hour study was estimated to be 652.63 mg/L on the basis of effects on growth inhibition.

Additional information

Short term toxicity to fish:

Experimental study result and predicted model data for the test chemical 1-methylpiperidin-4-ol (CAS No. 106-52-5) with respective read across were reviewed to summarize the following information:

In key study, Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) 1-methylpiperidin-4-ol. The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L LC50 (96 hours) Experimental = >100 mg/L LC100 (96 hours) (lowest loading at which 100% mortality was observed) = No mortality was observed. This value indicates that the substance 1-methylpiperidin-4-ol can be considered as not classified under short term toxicity to fish category.

In Supporting study, the 96 hrs aquatic toxicity study was predicted to assess toxic effects of the test compound 1-methylpiperidin-4-ol (CAS no. 106-52-5) and the result were predicted (SSS QSAR Prediction model, 2016). The study was based on the effects of the test compound on Danio rerio fish in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for the test compound was estimated to be 455.921020508 mg/l on the basis of mortality effect.

Whereas supporting model which is based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours LC50 was estimated to be 7885.35 mg/l on fish for substance 1-methylpiperidin-4-ol (CAS no. 106-52-5) with mortality effects.

Similarly 96 hrs LC50 value (Danish QSAR database, 2016) of test substance Methyl N-methylanthranilate onPimephales promelasfish was determined by three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database. Based on mortality effect of test organismPimephales promelas, the estimated 96 hrs LC50 value was evaluated to be 831.2391mg/l.

Above predicted lethal concentrations supported by experimental study for read across chemical 1-Methylpiperazine (Cas no. 109-01-3) which is indicates that in Preliminary assessment of acute fish toxicity of test substance 1-Methylpiperazine at test concentrations 0,683,1050,1620,2500 and 3850 mg/l was used with study condition 24.7 deg.C study temp., 39.8 mg/L CaCO3 hardness, 7.74 PH, 48.8 mg/L CaCO3 Alkalinity and 7.2 mg/L dissolved oxygen in static freshwater for 96 hrs. on fish species Pimephales promelas of 31 days age with 18.0 MM length and 0.081 G weight. During experiment the gas liquid chromatography was used for analytical detection. For data analysis the LC50 with corresponding 95% confidence intervals were calculated using the corrected average of analyzed tank concentration and the Trimmed Spearman-Karber Method used. On the basis of mortality effect the lethal concentration for test substance 1-Methylpiperazine was found to be 2300 mg/l. (Geiger,D.L.,et al; Centre for Lake Superior Environmental Studies, University of Wisconsin, Superior, WI:328 p;1986)

Another experimental data (SIDS INITIAL ASSESSMENT PROFILE, CoCAM2, Pg. no. 1-3, 17-19 April 2012) for read across N-Methyldiethanolamine (Cas no. 105-59-9) indicates the 96 h lethal concentrations for fish species Leuciscus idus as LC50= 1466 mg/L (nominal; static).

Thus, based on above experimental and predicted value from SSS QSAR, ECOSAR and Danish QSAR, and read across values it can be concluded that the substance 1-methylpiperidin-4-ol (CAS 106-52-5) can be considered as non toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Short term toxicity of aquatic invertebrate:
Various predicted studies and read across study for the 4-Piperidinol, 1-methyl-were reviewed to summarize the following information: 

48 hrs aquatic toxicity studies (EPI suite, ECOSAR version 1.1, 2016) were conducted to assess toxic effects of the 4-Piperidinol, 1-methyl- (CAS no106-52-5) and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for the 4-Piperidinol, 1-methyl- (CAS no106-52-5) was estimated to be 3674.980mg/l.

Using similar experimental conditions, 48 hr aquatic toxicity study (SSS QSAR prediction model, 2016) were conducted to assess toxic effects of the4-Piperidinol, 1-methyl- (CAS no106-52-5) and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the effective concentration (EC50) for the4-Piperidinol, 1-methyl- (CAS no106-52-5) was estimated to be 120 mg/L on the basis of intoxication.

A study was performed for read across chemical N-Methyldiethanolamine (105-59-9) as short term toxicity test (IUCLID dataset, European commission – European chemicals bureau,18–FEB–2000) in Daphnia magna for 48 hrs using guideline 79/831/EWG. The EC0, EC 50 and EC100 value in 48 hrs for short term toxicity to aquatic invertebrates for N-Methyldiethanolamine (105 -59 -9) were determined to be 125 mg/l, 230 mg/l and 500 mg/l respectively. Based on the result the N-Methyldiethanolamine was considered as not classified as per CLP regulation.

Another study on same read across cas was performed in Daphnia magna (Sids initial assessment profile, CoCAM2, 17-19 April 2012) for 48 hrs using guideline 79/831/EWG. From the experiment the EC0, EC 50 and EC100 value for short term toxicity to aquatic invertebrates for N-Methyldiethanolamine (105 -59 -9) were determined to be 250 mg/l, 400 mg/l and >500 mg/l. On the basis of result the N-Methyldiethanolamine was considered as not classified.

Based on the results of predicted data for target chemical and read across studies, it can be considered that the 4-Piperidinol, 1-methyl- (CAS No 106-52-5) is not likely to be toxic to aquatic invertebrates at environmentally relevant concentrations and can be considered as not classified as per the criteria of CLP regulation.

Toxicity of aquatic algae and cynobacteria:

Predicted data for the test chemical 4-Piperidinol, 1-methyl- (CAS No. 106-52-5) and the studies of its read across substance were reviewed to summarize the following information:

The key study is based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 1-methylpiperidin-4-ol (CAS no. 106 -52 -5). EC50 value was estimated to be 110.386 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 1-methylpiperidin-4-ol is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

Supporting the above data, QSAR prediction was done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours EC50 was estimated to be 1209.879 mg/l on green algae for substance 4-Piperidinol, 1-methyl- (CAS no. 106 -52 -5) with growth inhibition effects.

In an experimental study for RA CAS 105-59-9, Short term toxicity to Desmodesmus subspicatus study was carried out for 72 hrs (SIDS INITIAL ASSESSMENT PROFILE, CoCAM2, Pg. no. 17-19, April 2012). The test organism used for the study was Desmodesmus subspicatus. Based on growth rate of the test organism Desmodesmus subspicatus, the 72 hrs ErC50 value was determined to be 175.7 mg/l.

For same RA CAS 105-59-9 similar experimental Short term toxicity to Skeletonema costatum study was carried out for 72 hrs (SIDS INITIAL ASSESSMENT PROFILE, CoCAM2, Pg. no. 17-19, April 2012). The test organism used for the study was Skeletonema costatum. Based on growth rate of the test organism Skeletonema costatum, the 72 hrs ErC50 and EbC50 value was determined to be 410 and 110 mg/l, respectively.

Based on the overall reported results for target and read across substance, it can be concluded that the substance 4-Piperidinol, 1-methyl-can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Toxicity to microorganisms:
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the toxicity to microorganism was predicted for target substance 1-methylpiperidin-4-ol (CAS no. 106 -52 -5). IGC50 value was estimated to be 652.63 mg/l for Tetrahymena pyriformis for 48 h duration.