Reaction product of diazotised 4-amino-5-hydroxy-2,7- naphthalenedisulfonic acid coupled with resorcinol, subsequently coupled with diazotised 2-amino-4,6-dinitrophenol and 4-nitrobenzenamine, chelated with iron (3+), sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From July 17, 2000 to March 16, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

other: Royal Decree 363/1995 (BOE no. 133 of June 1995).

GLP compliance:

no

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Brachydanio rerio
- Source: P & S, S.L
- Length at study initiation: 2.75 ± 0.13 cm
- Weight at study initiation: 0.27 ± 0.1 g
- Feeding during test: no data

ACCLIMATION
- Acclimation period: 12 days
- Type and amount of food: commercial food
- Feeding frequency: 3 times per week up to 24 hours before the test.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

115 mg CaCO3/l

Test temperature:

22 ± 2 °C

pH:

8,09

Dissolved oxygen:

> 60%

Nominal and measured concentrations:

NOMINAL: 1 mg/l, 3.2 mg/l, 10 mglL, 32 mg/l and 100 mg/l
MEASURED: 1 mg/l, 3.21 mg/l, 10 mg/l, 32.1 mg/l and 100.4 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 3 l glass aquarium
- Type (delete if not applicable): closed
- Renewal rate of test solution: after 48 hours.
- Aeration: continuous aeration.
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1

OTHER TEST CONDITIONS
- Temperature: 22 ± 2 °C
- Photoperiod: 12 hours darkness.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Number of fish dead during the test period:

Concentration mg/l	Mortality
time	0h	24h	48h	72h	96h
Control	0	0	0	0	0
1	0	0	0	0	0
3.2	0	0	0	0	0
10	0	0	0	0	0
32	0	0	0	0	0
100	0	0	0	0	0

Conclusions:

The substance was tested for acute toxicity to fish following OECD 203. Under the experimental conidtions the LC50(96h) is more than 100 mg/L based on active ingredient.

Executive summary:

The substance has been tested for short-term toxicity testing on fish according to the guideline OECD 203 (1992) and Royal Decree operating method 363/1995 (BOE no.133 of June 1995).
The freshwater fish Brachydanio rerio has been subjected to the dye at test concentrations of: 1 mg/l, 3.21 mg/l, 10 mg /l, 32.1 mg/l
and 100.4 mg/l. The total test duration was 96 hours. The nominal LC50 (96 h) is more than 100 mg/L based on active ingredient.

Description of key information

LC50 (96 h) > 100 mg/l 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

OECD 203 (1992)