4-methylpyridine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1976

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-methylpyridine

EC Number:

203-626-4

EC Name:

4-methylpyridine

Cas Number:

108-89-4

Molecular formula:

C6H7N

IUPAC Name:

4-methylpyridine

Test material form:

gas under pressure: refrigerated liquefied gas

Details on test material:

- Name of test material: 4-Methylpyridine

Test animals

Species:

rat

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: Young animals, not previously used for testing purposes
- Weight at study initiation: 200-300 g
- Fasting period before study: 24 hours

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

Due to the small amount of the test material required it was measured and mixed with propylene glycol prior to being intubated via a syringe and
stainless steel catheter.

Doses:

125, 250, 500, 630, 800, 1000, 2000 mg/kg bw/day

No. of animals per sex per dose:

5 rats per sex per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

470 mg/kg bw

Based on:

test mat.

95% CL:

300 - 720

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

460 mg/kg bw

Based on:

test mat.

95% CL:

300 - 720

Clinical signs:

other: Lethargy and unkempt coats were noted in the animals dosed at levels ranging from 125 mg/kg bw - 500 mg/kg bw. Ocular hemorrhage accompanied lethargy and unkempt coats at 630 mg/kg bw and 800 mg/kg bw. Comas preceded death in the animals which succumbed.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

LD50 values for males and females were determined at 470 mg/kg bw and 460 mg/kg bw, respectively.