Registration Dossier
Registration Dossier
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EC number: 232-102-8 | CAS number: 7786-67-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to national standards. Study conducted in compliance with GLP regulations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isopulegol
- EC Number:
- 201-940-6
- EC Name:
- Isopulegol
- Cas Number:
- 89-79-2
- Molecular formula:
- C10H18O
- IUPAC Name:
- (1R,2S,5R)-2-Isopropenyl-5-methylcylohexanol
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany. The
inoculum was collected on 16 August 2010 from the aeration tank of the plant and aerated in
the laboratory until use. A suitable aliquot of the activated sludge suspension was sieved by a
finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon
in the blank controls the activated sludge was aerated with carbon dioxide free air for about
24 hours at 22 ± 2° C.
Aliquots of 7.5 mL were added to the test vessels to obtain a activated sludge concentration
of 30 mg/L dry weight. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 26 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 7 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10 - 20
- Sampling time:
- 8 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 70 - 80
- Sampling time:
- 17 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 80 - 90
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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