[8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Principles of method if other than guideline:

To determine octanol water partition coefficient of test chemical by HPLC method

GLP compliance:

no

Type of method:

HPLC method

Partition coefficient type:

octanol-water

Analytical method:

high-performance liquid chromatography

Key result

Type:

log Pow

Partition coefficient:

2.736

Temp.:

25 °C

Remarks on result:

other: test chemical is hydrophilic in nature.

Tables:

Reference substance	Log Pow	Rt1	Rt2	Rt3	Mean Rt ± S.D.	k	Log k
Formamide	NA	0.977	0.922	0.922	0.940±0.001	0	0
4-Methoxyphenol	1.3	1.108	1.107	NA	1.1075±0.001	0.1778	-0.7501
Phenol	1.5	1.15	1.149	NA	1.1495±0.001	0.2224	-0.6529
Benzene	2.1	1.771	1.775	NA	1.773±0.003	0.886	-0.0526
Biphenyl	4	2.822	2.841	NA	2.8315±0.013	2.011	0.3034
2-Nitrophenol	1.8	1.366	1.369	NA	1.3675±0.002	0.4543	-0.3427
Nitrobenzene	1.9	1.454	1.458	NA	1.456±0.003	0.5484	-0.2609
Toluene	2.7	2.103	2.104	NA	2.1035±0.001	1.237	0.0924
Ethylbenzene	3.2	2.519	2.527	NA	2.523±0.006	1.6831	0.2261
Naphthalene	3.6	2.388	2.393	NA	2.3905±0.004	1.5422	0.1881
n-Butylbenzene	4.6	4.454	4.463	NA	4.4585±0.006	3.741	0.573

Test substance		K	Log k	Log Pow by Generalized Calibration Graph	Mean of Log Pow ± S.D.
Rt1	1.794	0.909	-0.042	2.735	2.736± 0.001
Rt2	1.794	0.910	-0.041	2.736

Conclusions:

The partition coefficient (n-octanol/water) of test substance [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No.
68391-32-2) was determined as per the HPLC method (OECD Guideline-117). The Log Pow value was determined to be 2.736± 0.001 dimensionless at 25°C.

Executive summary:

The partition Coefficient n-octanol/water of [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride(CAS No.68391-32-2) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 117 for testing of Chemicals.

The reference substances were chosen according to functionally similarity with the test substance and calibration graph was prepared. The reference substances were 4-Methoxyphenol, Phenol, Benzene, Biphenyl, 2-Nitrophenol, Nitrobenzene, Toluene, Ethylbenzene, Naphthalene, n-Butylbenzene having Pow value ranging from 1.3 to 4.6 The Log Pow value was determined to be 2.736± 0.001.This value indicates that the substance is very hydrophilic in nature.

Description of key information

The partition coefficient (n-octanol/water) of test substance was determined as per the HPLC method (OECD Guideline-117). The Log Pow value was determined to be 2.736± 0.001 dimensionless at 25°C.

Key value for chemical safety assessment

Log Kow (Log Pow):

2.73

at the temperature of:

25 °C

Additional information

The partition Coefficient n-octanol/water of [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride(CAS No.68391-32-2) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 117 for testing of Chemicals. The reference substances were chosen according to functionally similarity with the test substance and calibration graph was prepared. The reference substances were 4-Methoxyphenol, Phenol, Benzene, Biphenyl, 2-Nitrophenol, Nitrobenzene, Toluene, Ethylbenzene, Naphthalene, n-Butylbenzene having Pow value ranging from 1.3 to 4.6 The Log Pow value was determined to be 2.736± 0.001.This value indicates that the substance is very hydrophilic in nature.