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REACH

2-(3,6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt

EC number: 235-440-4 | CAS number: 12227-78-0

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

GLP compliance:

not specified

Analytical monitoring:

not required

Vehicle:

Details on test solutions:

The test substance was soluble in water, In house solubility was found to be 652.61mg/L. Therefore the stock solution prepared as 1g/L, with the concentration of 1g/L, and was kept for 48hr stirring and 24hr settling period. After this filtered the stock and taken for experiment. From this stock solution further test concentrations were prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Age at study initiation (mean and range, SD): 3-4 months
- Length at study initiation (length definition, mean, range and SD): average 2.32 cm
- Weight at study initiation (mean and range, SD): 0.3038 g (approx.)
- Method of breeding:
- Feeding during test
- Food type:Standard brand feed,
- Amount:
- Frequency: once daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

151.2 mg of CaCO3

Test temperature:

23 - 24.33 °C

pH:

6.88

Dissolved oxygen:

8.0 mg/L

Nominal and measured concentrations:

6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 5liter plastic aquaria
- Type (delete if not applicable): closed
- fill volume: 2 liters
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 4 fishes
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12 hours light and 12-hour darkness
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24, 48, 72 and 96 hours of the start of the experiment.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality and symptoms

NominalConcentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h			48h		72h		96h
			V1	V2	V1	V2	V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	-	-	-	-	-		-	-	-	-	-	-	-
6.25	4	4	-	-	-	-	-		-	-	-	-	-	-	-
12.5	4	4	-	-	-	-	-		-	-	-	-	-	-	-
25	4	4	-	-	-	-	-		-	-	-	-	-	-	-
50	4	4	-	-	-	-	-		-	-	-	-	-	-	-
100	4	4	-	-	-	-	-		-	-	-	-	-	-	-

Visible Symptoms:

NominalConcentration

(mg/l)

Number of fish

Symptoms after

3 h

6 h

24h

48h

72h

96h

0 (control)

NS4

6.25

NS4

12.5

NS4

100

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 12227-78-0 on Zebra fish Danio rerio, was found to be >100 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical 2-(3, 6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. The test substance was soluble in water, In house solubility was found to be 652.61mg/L. Therefore the stock solution prepared as 1g/L, with the concentration of 1g/L, and was kept for 48hr stirring and 24hr settling period. After this filtered the stock and taken for experiment. From this stock solution further test concentrations were prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 2-(3, 6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was non hazardous and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. on Zebra Fish Danio rerio was determine to be >100 mg/l.

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 100 mg/L

Additional information

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