Registration Dossier
Registration Dossier
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Reaction mass of tetrasodium 8,8'-(1E,1'E)-(4,4'-(cyclohexane-1,1-diyl)bis(4,1-phenylene))bis(diazene-2,1-diyl)bis(7-hydroxynaphthalene-1,3-disulfonate) and trisodium 7-hydroxy-8-((E)-(4-(1-(4-((E)-(1-hydroxy-4-sulfonatonaphthalen-2-yl)diazenyl)phenyl)cyclohexyl)phenyl)diazenyl)naphthalene-1,3-disulfonate
EC number: 942-692-5 | CAS number: -
Endpoint summary
Administrative data
Description of key information
EC50 (48h) in aquatic invertebrates (Daphnia magna) = 48.9 mg/l.
ErC50 (7d) in aquatic plants (Lemna minor) = 50.3 mg/l.
Additional information
Justification for classification or non-classification
According to the CLP Regulation (EC 1272/2008), the threshold of classification of a substance for acute aquatic toxicity is 1 mg/l. This limit is compared with LC50 (96h) for fish, EC50 (48 h) for daphnia and EC50 (72 - 96 h) for algae or aquatic plants.
Available 48-hour test on fish could not be assessed due to the lack of details. Therefore, the classification process relied on data of toxicity to aquatic invertebrates and plants.
Toxicity on daphnia was assessed in a 48-hour study and acute immobilisation was seen in 50 % of daphnia exposed at concentration of 48.9 mg/l.
Toxicity on lemna was assessed in 7-day test and effects on growth rate of fronds and dry weight were reported with ErC50 = 50.3 mg/l and ErC50 = 251 mg/l, respectively.
No chronic toxicity data is available from long-term studies. In case of lack of chronic data, the potential for chronic toxicity is identified by appropriate combinations of acute toxicity data and lack of biodegradability. The threshold of classification is 100 mg/l.
Based on the results obtained with daphnia and lemna, classification for chronic aquatic toxicity was applied according to the CLP Regulation (EC 1272/2008).
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