1H-Pyrazole-5-carboxylic acid, 1-(3-chloro-2-pyridinyl)-4,5-dihydro-5-hydroxy-3-(hydroxymethyl)-, methyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to EU/OECD guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

methyl 1-(3-chloropyridin-2-yl)-5-hydroxy-3-(hydroxymethyl)-4,5-dihydro-1H-pyrazole-5-carboxylate

Cas Number:

1312304-36-1

Molecular formula:

C11H12ClN3O4

IUPAC Name:

methyl 1-(3-chloropyridin-2-yl)-5-hydroxy-3-(hydroxymethyl)-4,5-dihydro-1H-pyrazole-5-carboxylate

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

Immobilisation rates were recorded at 24-hour intervals. Additionally any abnormal behaviour or appearance of the Daphnia was reported.

Test conditions

Hardness:

The hardness of the dilution water used was 14.5 °dH (= 258.8 mg/L CaCO3).

Test temperature:

18 - 22 °C

pH:

During the test a temperature range of 18 - 22 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in each individual test.
The temperature, the pH and the oxygen values were measured every day in the freshly prepared media and in the media after 24 hours of exposure.

Dissolved oxygen:

The dissolved oxygen concentration remained above 3 mg/L throughout the exposure period.

Nominal and measured concentrations:

Nominal: 6.3, 12.5, 25, 50 and 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 51.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 51.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 51.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 51.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 51.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 51.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The results are expressed in terms of geometric mean measured concentrations.
Effective concentrations ranged from 87.8 % to 98.7 % in the freshly prepared media and from 4.1 %
to 29.1 % in the media after 24 hours of exposure.