Registration Dossier
Registration Dossier
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A mixture of isomers of: 1,1'-[(3,5(or 2,4 or 4,6 or 2,6)-dihydroxy-o(or m or p)-phenylene)bis(azo-meta-phenyleneazo{1-[3-(dimethylamino)propyl]-1,2-dihydro-6-hydroxy-4-methyl-2-oxopyridine-5,3-diyl})]dipyridinium-dichloride-dihydrochloride; 1-(1-[3-(dimethylamino)propyl]-5-{3-[x-(4-{1-[3-(dimethylamino)propyl]-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-5-pyridinio-3-pyridylazo}phenylazo)-2,4(or 2,6 or 3,5 or 4,6)-dihydroxyphenylazo]phenylazo}-1,2-dihydro-6-hydroxy-4-methyl-2-oxo-3-pyridyl)pyridinium-dichloride-dihydrochloride (where x is variable)
EC number: 404-540-1 | CAS number: 159405-95-5 BRAUN HM 2763; BROWN HM 2763; BRUN HM 2763; BRUNO HM 2763
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 (96h) = 115 mg/l, corresponding to 143 mg/l as nominal concentration.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 115 mg/L
Additional information
Stability of test item after 96 h in water was less than 80 %. Results were calculated on the basis of 80 % relative to the nominal concentrations of the test substance, instead of geometric mean of actually analysed concentrations as proposed in the test guideline. The test result still is considered to reflect the real situation with an appropriate approximation, thus the study all in all is considered as reliable with restrictions.
After 1.5 hours of exposure all fish had died at nominal 560 mg/l and after 6.5 hours 9 out of 10 fish had died at nominal 320 mg/l. At 96 hours of exposure 100 % mortality was seen at nominal 320 mg/l, 70 % at 180 mg/l and 10 % at 100 mg/l and no mortality at the other concentrations and the blank.
Analysis of the samples taken at the start (t = 0) revealed actual concentrations of 78 to 86 % at the start of the exposure. During the exposure period the actual test concentration decreased below 80 % relative to the nominal concentration. At 32 and 100 mg/l this decrease was 37 % and 28 %, respectively, possibly due to the active uptake and elimination of test substance by the fish exposed.
All results were related to actual concentrations of 80 % relative to the nominal concentrations.
Under test conditions, the test substance induces mortality at actual 80 (nominal 100) mg/l and higher, with an actual (nominal) 96h-LC50 of 115 (143) mg/l.
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