2-[(2-[2-(dimethylamino)ethoxy]ethyl)methylamino]ethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-07-02 to 1990-07-04

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

EEC Directive 67/548 Annex V C.2 as published in 84/449/EEC.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Texacat ZF-10, 2-[[2-[2-(dimethylamino)ethoxy]ethyl]methylamino]ethanol
- Physical state: Clear colorless liquid
- Analytical purity: 97%
- Lot/batch No.: 90/329
- Expiration date of the lot/batch: approximately two years from receipt (received on 30 March 1990)
- Storage condition of test material: dark at room temperature

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Each test concentration verified by chemical analysis (Control, 3.2, 10, 32, 100 and 320 mg/l)
- Sampling method: 100 ml water samples were taken from each test concentration at time 0 and 48 hours.
- Sample storage conditions before analysis: N/A

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in water

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Laboratory culture originating from astrain supplied by IRCHA, France.
- Age at study initiation (mean and range, SD): Less than 24 hours old
- Method of breeding: Culture conditions ensure reproduction is by parthenogenisis. Gravid adults were isolated 24 hours prior to test initation.
- Feeding during test
- Food type: mixture of fry fish food (Liquify(R)) and a suspension of mixed algae (predominantly Chlorella spp.)
- Amount: N/A
- Frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

CA 350 mg CaCO3/L

Test temperature:

21 ± 1 °C

pH:

7.8

Nominal and measured concentrations:

3.2, 5.6, 10, 18, 32, 56, 100, 180, and 320 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass jars
- Material, size, headspace, fill volume: 200 ml test solution
- Aeration: None
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated (with sodium thiosulphate) and aged laboratory tap water.


OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 150 - 200 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

72 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 59 - 88 mg/L

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Validity criteria fulfilled:

not specified

Conclusions:

The 24 and 48 hour EC50 were determined to be 180 mg/L and 72 mg/L respectively while the 24 and 48 hour NOECs were determined to be 100 mg/L and 18 mg/L, respectively.

Description of key information

The Acute Toxicity study of test substance to Daphnia magna was conducted according to OECD guidelines and followed GLP .

The 24 and 48 hour EC50 were determined to be 180 mg/L and 72 mg/L, respectively while the 24 and 48 hour NOECs were determined to be 100 mg/L and 18 mg/L, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

72 mg/L