Registration Dossier
Registration Dossier
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EC number: 234-364-9 | CAS number: 11120-25-5
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-07-01 to 2002-07-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Well documented, scientifically sound study that was conducted in accordance with GLP and the Commission Directive 88/302/EEC. The test method was similar to OECD guideline 209. However, there were no details on the testing conditions.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EC L133 Part C (1988)
- Principles of method if other than guideline:
- The method assesses the effect of a test substance on microorganisms by measuring the respiration rate under defined conditions in the presence of different concentrations of the test substance.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: Test concentration of the activated sludge 360.0 mg/L suspended solids
- Sampling method: Direct weighing - Vehicle:
- yes
- Details on test solutions:
- The test substance was added to about 130 mL deionized water and stirred overnight before testing (equilibration phase).
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Origin: aeration tank of waste water treatment plant treating predominantly domestic sewage (Wupper area water authority)
- Pretreatment: aeration of the activated sludge; daily feed with synthetic medium - Test type:
- other: mixed population of aquatic organisms (activated sludge)
- Water media type:
- not specified
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- 3 hours with permanent aeration
- Post exposure observation period:
- no data
- Hardness:
- no data
- Test temperature:
- 20 +/- 2 °C
- pH:
- pH of the suspension before application: 7.1
7.4 - 7.6 - Dissolved oxygen:
- 5.6 - 6.2
- Salinity:
- n/a
- Nominal and measured concentrations:
- Nominal concentrations: 100, 1000, 10000 mg/L
- Details on test conditions:
- no data
- Reference substance (positive control):
- yes
- Remarks:
- 3.5-Dichlorophenol, mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The test substance showed a 17.5% respiration inhibition of activated sludge at a test substance concentration of 10000 mg/L.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: See results below - Reported statistics and error estimates:
- no data
- Conclusions:
- The method assessed the effect of a test substance on micoorganisms by measuring the respiration rate under defined conditions in the presence of different concentrations of the test substance. Ammonium paratungstate showed 17.5% respiration inhibition of activated sludge at a test substance concentration of 10000 mg/L. The EC50 > 10000 mg/L.
Reference
Measured values of reference substance
| Test concentration (mg/L) | O2 start (mg O2/L) | O2 end (mg O2/L | Exposure time (min) | Temp. (C) | pH | |
| Reference | 5 | 6.4 | 4.8 | 4 | 20 | 7.7 |
| substance | 10 | 8.4 | 8.0 | 3 | 20 | 7.6 |
| 3.5 -Dichloro-phenol | 20 | 9.0 | 8.7 | 3 | 20 | 7.6 |
Results for Reference Substance: 3.5 -Dichlorophenol
| Test Concentration | Respiratory rate (mg/L.h) | Inhibition |
| 5 | 24.0 | 25.0 |
| 10 | 8.0 | 75.0 |
| 20 | 6.0 | 81.3 |
Description of key information
The method assessed the effect of a test substance on micoorganisms by measuring the respiration rate under defined conditions in the presence of different concentrations of the test substance. Ammonium paratungstate showed 17.5% respiration inhibition of activated sludge at a test substance concentration of 10000 mg/L. The EC50 > 10000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
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