2-dimethylaminoethyl methacrylate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Original report is not available. However, the report is cited in a peer-reviewed OECD SIDS. Hence, the reliability was taken from the OECD repor

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: National Institute for Environmental Studies (Japan)
- Age of parental stock (mean and range, SD): < 24 h
- Feeding during test
- Food type: Chlorella vulgaris
- Amount: 0.18 mg carbon/day/inividual
- Frequency: daily

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

29.5 - 39.3 mg/L

Test temperature:

20.4 - 21.0 °C

pH:

7.63 - 7.74 (control)
8.70 - 8.83 (63.2 mg/L)

Dissolved oxygen:

7.56 - 8.70 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 80 mL test solution in a 100 mL glass beaker
- Renewal rate of test solution (frequency/flow rate): every 24 h


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h light-darkness cycle
- Light intensity: < 1200 lux

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

4.35 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

7.86 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

16.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Measured concentrations:

Nominal conc. (mg/L)	Measured conc. (mg/L)
	0 d	1 d	7 d	8 d	14 d	15 d	20 d	21 d
	new	old	new	old	new	old	new	old
0.632	0.695	0.343	0.641	0.350	0.583	0.383	0.582	0.359
2.0	1.90	0.97	1.83	1.02	1.98	1.14	1.94	1.09
6.32	6.14	2.79	5.96	2.92	6.08	3.16	6.15	3.04
20.0	14.6	6.07	16.1	7.07	18.6	7.31	18.3	7.15
63.2	60.0	16.9	58.3	17.2	-	-	-	-

Cumulative number of juveniles produced per adult alive for 21 days.

	Nominal conc. mg/L
	(Measured conc. mg/L)
	control	0.632	2.0	6.32	20.0
	-	(0.479)	(1.44)	(4.35)	(11.2)
Mean (n=10)	67.8	67.6	57.2	62.1	7.9
S.D. (n=10)	10.1	11.0	10.5	12.1	6.4

In the highest test concentration (63.2 mg/L nominal) all parental animals were dead after 21 days.

Validity criteria fulfilled:

yes

Additional information

For 2-dimethylaminoethyl methacrylate, one GLP OECD guideline (211) study on long-term toxicity to aquatic invertebrates is reported. After 21 days of exposure a clear effect on the reproduction was measured (EC50 = 7.86 mg/L). The corresponding NOEC was determined at 4.35 mg/L.