Registration Dossier
Registration Dossier
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EC number: 215-676-4 | CAS number: 1341-49-7
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
No data are available for AMBI, however extrapolation is proposed from studies with other fluorides and ammoniium compounds. A number of non-guideline studies performed with sodium fluoride are available and give EC50 values of 26-48 mg/L. An LC50 of 101 mg/L is reported for total ammonia in Daphnia magna.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 26 mg/L
Additional information
No data are available for the substance, ammonium hydrogendifluoride (AMBI). The substance is water-soluble and, in aqueous environments, will dissociate to form hydrogen, fluoride and ammonium ions. Read-across data are available from other soluble fluoride salts and ammonia / ammonium compounds.
Fluoride studies
Camargo & Tarazona (1991) report that benthic larvae are sensitive to the concentration of fluoride, with EC50 values ranging from 26 -48 mg/L (actual concentration); however the study was performed in soft water. The EU RAR reviews and summairses the available data on short-term toxicity to aquatic invertebrates. The reported EC50 values for Daphnia sp. range from 97-352 mg/L and are based on nominal concentrations; EC50 values of 10.5 -39 mg/L are reported for marine invertebrates. The ICD summarises the available data on the short-term toxicity of sodium fluoride to aquatic invertebrates and reports EC50 values of 109 -340 mg/L; EC50 values of 30 -500 mg/L are reported for marine invertebrates.
Ammonia studies
The acute toxicity of ammonia was investigated in Daphnia magna. The 48 hour LC50 value for was determined to be 101 mg/L (with 95% confidence limits of 92.4 -110 mg/l) (Gersich & Hopkins 1986). A series of toxicity studies were conducted exposing either Chironomous tentans o rLumbriculus variegatus to various concentrations of ammonium chloride (Schubauer-Bergan et al, 1995). For non-ionised ammonia, the lowest LC50 to L. variegatus was 0.455 mg/L at a pH of 6.3. The lowest endpoint for C. tentans was 0.72 mg/L at a pH of 6.52. For total ammonia, the lowest LC50 to L. variegatus was 6.6 mg/L at a pH of 8.59. The lowest endpoint for C. tentans was 82.4 mg/L at a pH of 8.53. Total ammonia was more toxic to both species with increasing pH. The degree of pH dependence of toxicity, however, was more pronounced for L.variegatus. The smaller pH dependence of total ammonia toxicity to C. tentans suggest that ammonium ion contributes more significantly to the toxicity of total ammonia for this species.
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