ethyl 2-(4-phenoxyphenyl)lactate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPP 72-1 (Fish Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

429-220-9

EC Name:

-

Cas Number:

132584-17-9

Molecular formula:

C17 H18 O4

IUPAC Name:

ethyl 2-hydroxy-2-(4-phenoxyphenyl)propanoate

Test material form:

liquid

Specific details on test material used for the study:

Purity: 73.5%
Batch: IN-JG303-7

Sampling and analysis

Analytical monitoring:

yes

Remarks:

HPLC

Test solutions

Vehicle:

yes

Remarks:

DMF

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Dimethylformamide (DMF) at a maximum concentration of 0.1 ml
per liter.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Aquatic Research Organisms, Hampton, New Hampshire (eggs were purchased)
- Age at study initiation (mean and range, SD): After hatching, the fish were held in a 272-L, circular, fiberglass holding tank (84-cm diameter, 49-cm deep) for 76 to 84 days in continuously-flowing well water (mean temperature 9.7°C) at Haskell Laboratory
- Feeding frequency during acclimation: fish were fed live brine shrimp (Aquarium Products, Glen Burnie, Maryland) and PurinaÒ Trout ChowÒ (Purina Mills, Inc., St. Louis, Missouri) twice daily on weekdays, and once daily on weekends and holidays
- Health during acclimation (any mortality observed): Sickness, injury, and abnormalities were not present in fish in the holding tank nor in the fish used for testing

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

114 - 116 mg CaCO3/L

Test temperature:

11.3 - 12.0 deg C

pH:

7.0 - 7.8

Dissolved oxygen:

5.8 - 11.2 mg.L

Conductivity:

148 - 150 umhos/cm

Nominal and measured concentrations:

Nominal:0, 2.3, 3.9, 6.5, 10.8, and 18.0 mg/L
Measured: 0, 1.3, 2.4, 4.0, 6.3, and 12 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: stainless steel aquaria [30 (length) x 30 (width) x 30 (height) cm] which held approximately 15 L of test solution (26-L maximum volume; 17.0-cm test solution depth).
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Haskell Laboratory well water
- Total organic carbon: 0.45 mg/L
- Particulate matter: not reported
- Metals: not detected - 0.62 mg/L (lead)
- Pesticides: not detected
- Chlorine: not detected
- Alkalinity:
- Ca/mg ratio: 40.3/0.045

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours darkness with 30 minutes of transitional light
- Light intensity: 229-235 Lux (transitional light was 17-22 Lux)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and sub-lethal effects

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

7.2 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

7.2 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

6.9 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 4

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

6.7 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

12 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1.3 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

96-hr LC50 (rainbow trout) = 6.7 mg/L (confidence interval = 5.7 - 8.1 mg/L)