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EC number: 929-513-6 | CAS number: 1400584-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- EC Number:
- 929-513-6
- Cas Number:
- 1400584-86-2
- Molecular formula:
- C9H13N2O3 · Na
- IUPAC Name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- Test material form:
- solid: bulk
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 49.0 mg of the test item were dissolved in Milliporewater and made up with Milliporewater to the mark in a 100 mL volumetric flask to prepare a stock solution of 490 mg/L. Defined volumes of this stock solution were diluted with Milliporewater to obtain standard solutions in the range of 0.025 to 4.90 mg/L.
Sample storage conditions before analysis:
Routinely, the samples are analysed immediately. Only in exceptional cases, they are stored overnight deeply frozen and protected from light.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- To produce the only test concentration 125.0 mg test item were added to 1 litre of dilution water. The pH was adjusted to pH 7.9.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Common name: green algae
Source: Non-axenic strain of the test species obtained from "The Collection of Algal Cultures" of the Institute of Plant Physiology at the University of Gottingen (Germany).
Maintenance of stock cultures:
Exponentially-growing stock cultures are maintained in the test facility under constant temperature conditions (23 +/- 2°C) at a light intensity in the range 60 - 120 µE x m-2 x s-1 (measured in the range 400 to 700 nm using a spherical quantum flux meter). The nutrient medium (according to BRINGMANN & KUHN (1977) is renewed once a week. Cell density measurements are made using a microcell counter.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Water hardness of the final nutrient medium is 1.3°dH, corresponding to 22.5 mg/L CaCO3.
- Test temperature:
- 21 - 25 °C
- pH:
- 8.1 - 9.6
- Nominal and measured concentrations:
- nominal: 100 mg/L
meassured: 99.5 mg/L (0 h), 99.9 mg/L (48 h) - Details on test conditions:
- Exposure conditions
Test vessels: 300 mL Erlenmeyer flasks with cotton stoppers
Culturing apparatus:
Light chamber in which a temperature in the range 2 °C to 25 °C can be maintained at +/- 2 °C, and continuous uniform illumination is provided in the spectral range 400 to 700 nm.
Light intensity:
At the average of the test solutions, a light intensity in the range 60 to 120 µE x m-2 x s-1, or in equivalent range of 4000 to 8000 lx, is recommended to use.
Cell density measurements:
Cell densities are measured in a microcell counter or, alternatively, are determined by means of a microscopic counting chamber.
Experimental design:
1 test concentration plus 1 control
3 replicates per concentration, 6 replicates per control
initial cell density in the test cultures approximately 5000 cells per millilitre
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No toxic effects against algae at a limit test concentration of 100 mg/L.
- Executive summary:
A study was performed to assess adverse effects on the growth (= increase in cell density) and the growth rate (= rate of increase in cell density with time) of the planktonic freshwater algal species Desmodesmus subspicatus over several generations.
The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 3 'Algal inhibition test' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test'.
Exponentially growing algal cells were exposed for a period of 72 hours to a limit test concentration of nominally 100 mg/L dissolved in water.
The cell densities were measured at 24-hour intervals. Inhibition of the algal population was measured as reduction in growth (index b) and growth rate (index r), relative to control cultures grown under identical conditions. The following values were determined:
Results
[mg/L]
ErC 50 (0-72 h)
> 100
EbC 50 (0-72 h)
> 100
No toxic effects against algae at a limit test concentration of 100 mg/L.
All results are expressed in terms of nominal concentrations. Recovery rates correspond to 99.5 % of nominal values at 0 hours, and to 99.9 % of nominal values at 48 hours, respectively.
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