Heptadecanol, branched and linear

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

GLP compliance:

yes (incl. QA statement)

Remarks:

testing lab.

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Heptadecanol, branched and linear

EC Number:

291-378-8

EC Name:

Heptadecanol, branched and linear

Cas Number:

90388-00-4

Molecular formula:

C17H36O

IUPAC Name:

2-methylhexadecan-1-ol; heptadecan-1-ol

Details on test material:

Name of the test substance used in the study report: HEPTADECANOL N
Purity: 99.8%

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

Age when treated: 11 weeks
Body weight when treated: 180 - 189 g
The rats were identified using unique cage number and corresponding color-coded spots on the tail.
The animals were housed in air-conditioned rooms with a room temperature of 22 +/°C and a relative humdity between 30 - 70%. The day/night rhythm was 12 h light and 12 h dark.
Three animals per cage (type Makrolon 4 with wire mesh tops and standard softwood bedding).
The rats were offered a standardized laboratory diet as well as tap water ad libitum.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

VEHICLE:
Yellowish oily liquid
Batch Number: 24788186
Source: Carl Roth GmbH & Co., 76185 Karlsruhe / Germany
Stability: Stable under storage conditions
Expiry Date: 30-Nov-2012
Storage Conditions: At room temperature (range of 20 ± 3°C), light protected.
Safety Precautions: Routine hygienic procedures were used to ensure the health and safety of the personnel.
Olive oil was found to be a suitable vehicle.

TEST ITEM:
Dosing volume: 10 m l/kg b.w.

Doses:

2000 mg/kg

No. of animals per sex per dose:

6 (2 test groups)

Control animals:

no

Details on study design:

Clinical signs: Daily during the acclimatization period, during the first 30 min and approx. 1, 2, 3 and 5 h after administration on test day 1, depending on the occurrence of clinical signs of toxciity. Once daily during days 2 - 15.
Body weight: On tst day 1 (prior to administration), 8 and 15.
Viability/Mortality: Daily during the acclimatization period, during the first 30 min and at approx. 1, 2, 3 and 5 h after administration on test day 1 (in common with the clinical signs) and twice daily during days 2 - 15.
Necropsy: All animals were killed at the end of the observation period by carbon dioxide asphyxiation and discarded after macroscopic examinations were performed. No organs or tissues were retained.
Observation period: 15 days

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: no deaths

Mortality:

none

Clinical signs:

other: none

Gross pathology:

Nothing abnormal detected.