[No public or meaningful name is available]

Genetic toxicity: in vitro

Administrative data

Endpoint:

genetic toxicity in vitro, other

Remarks:

mammalian cell gene mutation assay; sister chromatid exchange assay in mammalian cells; DNA damage and repair assay in mammalian cells

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1990

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The genotoxicity of lead acetate was investigated in the presence or absence of UV irradiation. A DNA strand break assay in HeLa cells was used to examine DNA damage associated with exposure to 500 uM lead acetate. In V79 cells, 0.5 to 5 uM lead acetate was used in a HPRT locus mutation assay and 1 to 10 uM lead acetate was used in a sister-chromatid exchange (SCE) assay.

GLP compliance:

not specified

Type of assay:

other: mammalian cell gene mutation assay; sister chromatid exchange assay in mammalian cells; DNA damage and repair assay in mammalian cells

Test material

Test material information

Constituent 1

Reference substance name:

Acetic acid, lead salt, basic

EC Number:

257-175-3

EC Name:

Acetic acid, lead salt, basic

Cas Number:

51404-69-4

Results and discussion

Test results

Species / strain:

other: Human HeLa cells; Chinese hamster lung fibroblasts (V79)

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

not examined

Remarks on result:

other: Test system: all strains/cell types tested

Any other information on results incl. tables

Lead acetate alone did not induce DNA strand breaks, but UV-induced strand breaks persisted longer in the presence of lead acetate compared to UV-irradiation alone. Lead acetate alone did not induce mutations at the HPRT locus, but did enhance UV-induced mutagenicity between 0.5 and 5 uM. Lead acetate alone did not induce an increase in SCEs, but did enhance the number of UV-induced SCEs at all concentrations tested.

Applicant's summary and conclusion

Conclusions:

Interpretation of results: negative

Executive summary:

The genotoxicity of lead acetate was investigated in the presence or absence of UV irradiation. A DNA strand break assay in HeLa cells was used to examine DNA damage associated with exposure to 500 uM lead acetate. In V79 cells, 0.5 to 5 uM lead acetate was used in a HPRT locus mutation assay and 1 to 10 uM lead acetate was used in a sister-chromatid exchange (SCE) assay. Lead acetate alone did not induce DNA strand breaks, but UV-induced strand breaks persisted longer in the presence of lead acetate compared to UV-irradiation alone. Lead acetate alone did not induce mutations at the HPRT locus, but did enhance UV-induced mutagenicity between 0.5 and 5 uM. Lead acetate alone did not induce an increase in SCEs, but did enhance the number of UV-induced SCEs at all concentrations tested.