L-Glutamine, L-alanyl-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-07-21 to 1994-07-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Adopted 4 April, 1984

Deviations:

yes

Remarks:

Test concentrations exceeded recommended highest concentration of 1 g/L

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all concentrations, freshly prepared and aged test solution after 0, 24 and 48 h.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 2.5 g test substance to 250 mL solution water. The test concentrations were prepared separately by diluting the stock solution.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): 6 - 24 h
- Source: bred at testing facility originally obtained from LWA Düsseldorf, Germany
- Feeding during test: no

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.0 - 21.0 °C

pH:

6.17 - 7.86

Dissolved oxygen:

6.5 - 8.7 mg/L

Nominal and measured concentrations:

Nominal test substance concentrations: control, 4096, 8192 and 10000 mg/L
The recovery rate was > 90%.

Measured at 0 h: 3953, 7941 and 9692 mg/L (new media)
Measured at 24 h: 3913, 8461 and 9830 mg/L (old media); 4179, 8373 and 9990 (new media)
Measured at 48 h: 4077, 8291 and 10226 mg/L (old media)

Details on test conditions:

TEST SYSTEM
- Type (delete if not applicable): covered with paper
- Volume of solution: 10 mL
- Renewal rate of test solution: after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted test water (ISO test water)
- Intervals of water quality measurement: at test start and after 24 h and 48 h

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night cycle

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilisation after 0, 24 and 48 h

RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100, 1000 and 10000 mg/L
- Results used to determine the conditions for the definitive study: no immobilised Daphnia after 48 h

Reference substance (positive control):

yes

Remarks:

potassium-dichromate

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

8 192 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: no

Results with reference substance (positive control):

Tested once per month, results of the latest testing: EC50 (48 h): 1.4 mg/L

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this test procedure a acute toxic effect of the test substance towards daphnia was found after 48 hours in the test solution of 10 g/L resulting in an EC50 (48 h) of 10000 mg/L.

Description of key information

EC50 (48 h): 10000 mg/L (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

10 000 mg/L

Additional information

One study investigating the acute toxicity of the substance to aquatic invertebrates is available. The study was conducted according to OECD guideline 202, part I (1984, GLP). Daphnia magna were exposed to nominal test substance concentrations of 4096, 8192 and 10000 mg/L incl. a control. The test solutions were renewed after 24 hours and the test concentration was analytically verified by HPLC. The recovery rates in the freshly prepared and in the aged test solutions were >90%. Thus, the effect concentrations were based on nominal concentrations. An immobilisation of 50% was observed at the highest test concentration of 10000 mg/L after 48 hours. Based on this result an EC50 (48 h) of 10000 mg/L was determined.