1-Methyl-1-propylpyrrolidinium Bis(fluorosulfonyl)imide

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020-12-18 to 2021-02-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

See attached report

Vehicle:

no

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

The Pseudokirchneriella subcapitata stock culture was maintained at 4°C and regularly transferred to fresh medium at the laboratory. The algal stock culture was provided by the CCAP (Culture Collection of Algae and Protozoa), reference CCAP 278/4.
A pre-culture was prepared from the stock culture and maintained in an exponential growth by successive transfers to fresh medium used in the test (OECD medium) every 3 to 4 days. To ensure that the algae are in the exponential growth phase when used to inoculate the test solutions, an inoculum culture in the test medium was prepared 3 days before start of the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

23 to 23.7°C

pH:

7.7 to 8.1

Nominal and measured concentrations:

Nominal: 0; 6.25; 12.5; 25.0; 50.0; 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of an experimental study performed according to OECD 201 guideline and under GLP conditions, the substance exhibits EC50 and EC10 above 100 mg/L on the growth of Pseudokirchneriella subcapitata over a period of 72 hours.

Executive summary:

The objective of this study was to assess the effects of test item N-Propyl-N-methylpyrrolidinium Bis(fluorosulfonyl)imide on the growth of Pseudokirchneriella subcapitata over a period of 72 hours according to OECD 201 Guideline. Pseudokirchneriella subcapitata is a freshwater green algae that is recommended by the OECD Guideline 201 (2006, corrected 2011).

The test item is a soluble product under the test conditions and the test solutions were prepared in the test medium by dilution of a primary stock solution of test item.

The effects of N-Propyl-N-methylpyrrolidinium Bis(fluorosulfonyl)imide at the definitive nominal concentrations of 0; 6.25; 12.5; 25.0; 50.0 and 100 mg test item.L-1 to Pseudokirchneriella subcapitata were investigated under laboratory static conditions.

The analytical samples were sampled at the beginning of the test and at the end of the test (72 hours) with the content of the vessels pooled and homogenized. Since the deviations between the measured concentrations and the nominal concentrations were within the range of ± 20 %, analysis of the results was based on the nominal concentrations.

The results are the following:

NOEC (growth rate): 100 mg/L

LOEC (growth rate): > 100 mg/L

NOEC (yield): 100 mg/L

LOEC (yield): > 100 mg/L

72h-ErC10: > 100 mg/L

72h-ErC20: > 100 mg/L

72h-ErC50: > 100 mg/L

72h-EyC10: > 100 mg/L

72h-EyC20: > 100 mg/L

72h-EyC50: > 100 mg/L

Description of key information

Under the conditions of an experimental study performed according to OECD 201 guideline and under GLP conditions, the substance exhibits EC50 and EC10 above 100 mg/L on the growth of Pseudokirchneriella subcapitata over a period of 72 hours.

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L

EC10 or NOEC for freshwater algae:

100 mg/L

Additional information

The validity criteria of the study are met. Therefore, we can consider this study valid and acceptable for that endpoint.