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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental started 16th January 2017 . Experimental completed 18th January 2017. Final report issued 10th February 2017.
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
hexasodium 5,5'-{[2,7-disulfonato-9-(2-sulfonatophenyl)-9H-xanthene-9-ylium-3,6-diyl]bis(azanediyl)bis[(2,4,6-trimethyl-3,1-phenylene)aminosulfonyl]}diisophthalate
EC Number:
854-560-3
Cas Number:
1891094-25-9
Molecular formula:
C53H38N4Na6O22S5
IUPAC Name:
hexasodium 5,5'-{[2,7-disulfonato-9-(2-sulfonatophenyl)-9H-xanthene-9-ylium-3,6-diyl]bis(azanediyl)bis[(2,4,6-trimethyl-3,1-phenylene)aminosulfonyl]}diisophthalate
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Species :Daphnia Magna clone A
Sourced - University of Sheffield UK, date of Supply 9th July 1990
Selection of daphids - Daphidds less than 24 hours were selected for the study
Acclimatisation
Young daphids produced by parents cultured in this laboratory were used . The parents to obtains the young daphids were bred in the same quality of water (dechlorinated tap water)
water temperature 20°C +/- 1°C and photoperiod (16hours light/ 8 hours dark)
Chlorella vulgaris 0.1-0.2 mgC (organic carbon content )/ day per Daphnia was fed to the parents once a day.

Aloocation of test groups -:- random selection

Study design

Test type:
static
Water media type:
other: dechlorinated water
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
Total hardesss as CaCO3 (28mg/l)
Test temperature:
20°C +/- 1°C
pH:
pH 7.8-7.9
Dissolved oxygen:
more than 3mg/l before aeration. Measured oxygen 8.7 at the start . 8.8 at the end .
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 was determined to be >100mg/l (the concentration based on nominal concentration).
Executive summary:

Introduction

The objective of this study is to determine the 48 hour median effective concentration (EC50) by conducting an acute immobilsation study of the test susbstance in daphnia magna following OECD guidance for the testing of chemicals No 202, April 13, 2004 "daphnia sp.,acute immobilisation test".

Results and conclusion

The 24 hour and 48 hour EC50 was determined to be >100mg/l (the concentration based on nominal concentration).