Reaction mass of disodium 2-({4-methyl-2-[(4-{[5-methyl-1-(2-sulfonatophenyl)hexan-3-yl]amino}-9,10-dioxo-9,10-dihydroanthracen-1-yl)methyl]pentyl}amino)benzene-1-sulfonate,disodium 4-{5-methyl-3-[(4-{[5-methyl-1-(4-sulfonatophenyl)hexan-3-yl]amino}-9,10-dioxo-9,10-dihydroanthracen-1-yl)amino]hexyl}benzene-1-sulfonate anddisodium 4-{5-methyl-3-[(4-{[5-methyl-1-(2-sulfonatophenyl)hexan-3-yl]amino}-9,10-dioxo-9,10-dihydroanthracen-1-yl)amino]hexyl}benzene-1-sulfonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 Jan to 03 Mar 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Disodium [(9,10-dihydro-9,10-dioxo-1,4-anthrylene)bis(imino-4,1-phenyleneoxy)]bis(benzenesulphonate)

EC Number:

274-355-7

EC Name:

Disodium [(9,10-dihydro-9,10-dioxo-1,4-anthrylene)bis(imino-4,1-phenyleneoxy)]bis(benzenesulphonate)

Cas Number:

70161-19-2

Molecular formula:

C38H26N2O10S2.2Na

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

All test medium samples for the analytical determinations were taken after mixing of the test solutions from the approximate center of the test vessels and kept at -18°C to -25 °C until analyses.

Test solutions

Vehicle:

no

Details on test solutions:

Stock solution: 100.4 mg test substance were mixed and made up to 1000 ml with water.
Test concentrations: dilution of stock solution with reconstituted water

Calculated amounts of the stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. After an equilibration time of 7 hours the daphnia were transferred into the beakers. The test substance was homogeneously distributed in the test vessels at all test concentrations at the begin of exposure. At the end of exposure a deposit was observed at all test concentrations and small parts of the substance were swimming at the surface of the test water at concentrations 21, 46 and 100 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organism was the species Daphnia magna. The daphnids were bred in the laboratories of the Textile Dyes Division, Product Ecology TF under standardized conditions. For the test, animals with age of less than 24 hours were used. The quality of the daphnids will be checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8 -1.5 mg/l).
For each concentration, 20 Daphnia magna were used.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

240 mg/L CaCO3 (dilution water)

Test temperature:

21 - 22°C

pH:

7.8 - 8.0

Dissolved oxygen:

101 - 102%

Nominal and measured concentrations:

Nominal: 0 (control), 4.4, 9.6, 21, 46, 100 mg/L
Measured (48 h): <2.0 (control), 7.0, 9.2, 11, 16, 32 mg/L

Details on test conditions:

Vessels: Beakers covered with watch glasses 100 mL solution per beaker
Water: Reconstituted water
Temperature: 20 +/- 1°C
Lighting: 16 h light, approx. 1500 lux

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 32 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: measured end concentration after 48 h

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: yes, in three highest concentrations

Reported statistics and error estimates:

N/A

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 to Daphnia magna was determined to be >32 mg/L (measured) which corresponds to 100 mg/L (nominal). The substance is not acutely harmul to aquatic invertebrates up to the maximum water solubility in the test medium.