Sodium 2-amino-4,6-dinitrophenoxide

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two hydrolysis studies as-
2. and 3.

GLP compliance:

not specified

Radiolabelling:

not specified

Analytical monitoring:

yes

Remarks:

and 3. not specified

Buffers:

2.
Composition of buffer:
pH 4: 0.4 mL of 0.1 mol/L NaOH and 10 mL of 0.5 mol/L potassium acid phthalate were filled up to 100 mL with purified water.
pH 7: 29.6 mL of 0.1 mol/L NaOH and 10 mL of 0.5 mol/L monopotassium phosphate were filled up to 100 mL with purified water.
pH 9: 21.3 mL of 0.1 mol/L NaOH, 10 mL of 0.5 mol/L H3BO3 in 0.5 mol/L KCL were filled up to 100 mL with purified water.

3. No data available

Duration:

5 d

Temp.:

50 °C

Initial conc. measured:

0.05 mg/L

Remarks:

2. The study was performed at pH 4, 7 and 9, respectively.

Temp.:

50 °C

Remarks:

3. Other details not known

Positive controls:

not specified

Negative controls:

not specified

Transformation products:

not specified

Temp.:

50 °C

Remarks on result:

other: 2. The study was performed at pH 4, 7 and 9, respectively. Although the half-life value of test chemical was not, but the residues of the test chemical were more than 90 % in all the pH. Thus, the test chemical was reported to be stable in water.

Temp.:

50 °C

DT50:

> 1 yr

Remarks on result:

other: Other details not known

Details on results:

2. The residues of the test chemical were more than 90 % in all the pH. Thus, the test chemical was reported to be stable in water.

3. The half-life value of test chemical was determined to be > 1 yr at a temperature of 50⁰C.

2.

pH	Initial concentration	Concentration after 5 days	Residue	Average residue
pH 4.0	10.7 mg/L	10.8 mg/L	100 %	100 %
		10.8 mg/L	100 %
pH 7.0	10.6 mg/L	10.8 mg/L	102 %	102 %
		10.8 mg/L	102 %
pH 9.0	10.7 mg/L	10.5 mg/L	98.5 %	98.5 %

3. No data available

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the hydrolysis half-life value of the test chemical can be expected to be > 1 yr, at pH range 4, 7 & 9 and a temperature of 50°C, respectively. Thus, based on this half-life value, it can be concluded that the test chemical is not hydrolysable in water.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the half-life of the test chemical.The studies are as mentioned below:

The half-life of the test chemical was determined at different pH range.The study was performed according to OECD Guideline 111 (Hydrolysis as a Function of pH) at a temperature of 50°C.Test substance was added in the test water of pH 4, pH 7 and pH 9 to be the test concentration of about 0.05 mg/L. These test solutions were shaked for 5 days at 50 degree C. After that, the test chemical concentration of each test water was measured and compared with the initial concentration. Analytical method involve the use of HPLC. L-column ODS (15 cm x 4.6 mm I.D.) was used as a column for the study.Although the half-life value of test chemical was not known, but the residues of the test chemical were more than 90 % in all the pH. Thus, the test chemical was reported to be stable in water at a temperature of 50⁰C and at pH 4, 7 and 9, respectively. Based on the half-life values, it is concluded that the test chemical is not hydrolysable.

For the test chemical, the half-life of the test chemical was determined.The study was performed at a temperature of 50⁰C. Test chemical was reported to be hydrolytically stable with a corresponding half-life value of> 1 yrat a temperature of 50⁰C, respectively.Thus, based on the half-life value, it is concluded that the test chemical is not hydrolysable in water.

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the hydrolysis half-life value of the test chemical can be expected to be > 1 yr, at pH range 4, 7 & 9 and a temperature of 50°C, respectively. Thus, based on this half-life value, it can be concluded that the test chemical is not hydrolysable in water.

Description of key information

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the hydrolysis half-life value of the test chemical can be expected to be > 1 yr, at pH range 4, 7 & 9 and a temperature of 50°C, respectively. Thus, based on this half-life value, it can be concluded that the test chemical is not hydrolysable in water.

Key value for chemical safety assessment

Half-life for hydrolysis:

1 yr

Additional information

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the half-life of the test chemical. The studies are as mentioned below:

 

The half-life of the test chemical was determined at different pH range. The study was performed according to OECD Guideline 111 (Hydrolysis as a Function of pH) at a temperature of 50°C.Test substance was added in the test water of pH 4, pH 7 and pH 9 to be the test concentration of about 0.05 mg/L. These test solutions were shaked for 5 days at 50 degree C. After that, the test chemical concentration of each test water was measured and compared with the initial concentration. Analytical method involve the use of HPLC. L-column ODS (15 cm x 4.6 mm I.D.) was used as a column for the study. Although the half-life value of test chemical was not known, but the residues of the test chemical were more than 90 % in all the pH. Thus, the test chemical was reported to be stable in water at a temperature of 50⁰C and at pH 4, 7 and 9, respectively. Based on the half-life values, it is concluded that the test chemical is not hydrolysable.

 

For the test chemical, the half-life of the test chemical was determined. The study was performed at a temperature of 50⁰C. Test chemical was reported to be hydrolytically stable with a corresponding half-life value of> 1 yrat a temperature of 50⁰C, respectively. Thus, based on the half-life value, it is concluded that the test chemical is not hydrolysable in water.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the hydrolysis half-life value of the test chemical can be expected to be > 1 yr, at pH range 4, 7 & 9 and a temperature of 50°C, respectively. Thus, based on this half-life value, it can be concluded that the test chemical is not hydrolysable in water.